宁夏羊角椒雄性不育与膜脂过氧化和保护酶的关系初步研究

Study on the Relationship Between Pepper(Ningxia Cavel) Male Sterility Line and the Membrane Lipid Peroxidation and Protective Enzymes

以宁夏农林科学院种质资源研究所辣椒育种课题组发现的田间自然突变的宁夏羊角椒雄性不育株系和其可育株系为试验对象,对其叶片和不同发育阶段的花蕾的膜脂过氧化产物丙二醛(Malondialdehyde,MDA)的含量和保护酶过氧化物酶(Peroxidase,POD)、超氧化物歧化酶(Supper oxide,SOD)和过氧化氢酶(Catalase,CAT)的活性进行研究。结果表明:宁夏羊角椒雄性不育株系不同发育阶段的花蕾和叶片中MDA含量、POD和SOD活性都高于可育株系,第1发育阶段差距显著;但不育株系花蕾发育中后期和叶片中的CAT活性则低于可育株系。从试验结果可以看出,花蕾发育前期膜脂过氧化程度过高,是导致宁夏羊角椒雄性不育株系花粉败育的重要因素之一。

With a new pepper （Ningxia Cavel）male sterility line and its fertility line discovered by researchers in Germplasm Resources of Ningxia Academy of Agriculture and Forestry Sciences as materials. The content of MDA produced by the membrane lipid peroxidation, the activities of POD, SOD and CAT in different bud＇s stage and the leaves of male sterility line and its fertility line were studied. The results showed that,the content of MDA and the activities of POD and SOD in different bud＇s stage and the leaves of male sterility line was higher than its fertility line, but the activity of CAT in the last two bud＇s stage and the leaves of male sterility resource was lower than its fertility line. This study showed that pepper（Ningxia Cavel）male sterility linewas caused by the membrane lipid peroxidation.