摘要
为实现高信噪比(SNR)的深层生物组织成像,结合了激光扫描共聚焦成像技术和近红外(NIR)荧光成像技术,根据近红外荧光成像要求设计了一套激光扫描共聚焦近红外荧光成像实验系统,对注入近红外荧光染料LDS925小鼠的尾部成像后获得了小鼠尾部近红外荧光图像和近红外共聚焦荧光图像。实验结果表明小鼠尾部近红外共聚焦荧光图像信噪比显著优于小鼠尾部近红外荧光图像,采用均方差和峰谷(PV)值进行评估时,近红外荧光成像荧光信号强度分布的均方差值和PV值分别为864和102;共聚焦荧光成像的荧光信号强度分布的均方差值和PV值分别为1459和255;进一步表明激光扫描共聚焦成像技术在近红外荧光成像中应用是可行的,可以实现深层组织的高信噪比共聚焦成像。
In order to obtain high signal- to- noise ratio（SNR） tissue images with deeper imaging depths, the laser scanning confocal technology with the near infrared（NIR） fluorescence imaging is integrated and a laser scanning confocal NIR fluorescence imaging system based on requirements of NIR fluorescence imaging is established. A laboratory mouse injected with NIR fluorescence labeling LDS925 is placed in this system and a non-confocal NIR fluorescence image and a confocal NIR fluorescence images for the tail of laboratory mouse is obtained. The experimental results show that when evaluated by the mean square error and the peak valley（PV） value, the mean square error and the PV value is 864 and 102 respectively for the non- confocal NIR fluorescence image and 1459 and 255 for the confocal NIR fluorescence image, which further shows that the laser scanning confocal technology is applicable to the NIR fluorescence imaging, and the in vivo and high SNR tissue imaging with deeper imaging depths can be achieved.
出处
《激光与光电子学进展》
CSCD
北大核心
2014年第11期190-195,共6页
Laser & Optoelectronics Progress
基金
国家重大科研装备研制项目(ZDYZ2013-1)
苏州市应用基础研究计划(SYG201324)
