柴黄片超高效液相特征指纹图谱研究

Study on UPLC fingerprint of Chaihuang tablets

目的:建立柴黄片超高效液相特征指纹图谱,为柴黄片的质量控制提供依据。方法:采用Acquity UPLC BEH C18(2.1 mm×100 mm,1.7μm)色谱柱;柱温40℃;检测波长280 nm;流动相为乙腈和0.2%冰醋酸梯度洗脱,流速0.3 ml·min-1,进样体积3μl。结果:超高效液相特征指纹图谱中主要成分得到有效分离,得到13个共有峰,各批次柴黄片特征指纹图谱与对照图谱相似度均在0.9以上,可用于柴黄片的定性鉴别。结论:建立了柴黄片的特征指纹图谱,此方法简单、准确、重复性良好,为柴黄片的质量控制提供了有效的方法。

OBJECTIVE To establish a method of UPLC fingerprint for Chaihuang tablets so as to provide basis for Chaihuang tablets quality control. METHODS The Acquity UPLC BEH C18（2. 1 min× 100 mm, 1.7μm） column was adopt- ed,the column temperature was controlled at 40 ℃, and the detection wavelength was 280 nm. The mobile phase for UPLC fingerprint establishment was acetonitrile -0. 2 % acetic acid solution with gradient elution at a flow rate of 0. 3 mL· min^- 1 and the injection volume was 3 I11. RESULTS The major components in UPLC fingerprints could be separated effectively, 13 com- mon peaks were obtained in reference fingerprint. Good similarities with correlation coefficients higher than 0. 9 were found in fingerprints between samples and standard fingerprint, which could be utilized for the identification of ChaiHuang tablets. CONCLUSION The analytical method of UPLC fingerprint of Chaihuang tablets was established. The method is accurate and simple, which can be used as a quality control method for Chaihuang tablets.