枸杞多糖对肝癌模型小鼠肿瘤抑制与免疫功能的影响

Effects of Lycium chinensis Polysaccharides on Tumor Suppression and Immune Function of Liver Cancer Model Mice

目的:研究枸杞多糖对肝癌模型小鼠肿瘤抑制与免疫功能的影响。方法:于小鼠腋窝皮下接种H22瘤株以复制小鼠肝癌模型。20只KM小鼠随机均分为正常对照(等容生理盐水)组、模型(等容生理盐水)组、环磷酰胺(20 mg/kg)组、枸杞多糖(5mg/kg)组,灌胃给药,每天1次,连续10 d。测定小鼠体质量、抑瘤率、血清肿瘤标记物、炎症因子水平。结果:与正常对照组比较,模型组小鼠至给药第5天起体质量减少,给药10 d后瘤质量增加,小鼠血清甲胎蛋白(AFP)、癌胚抗原(CEA)、肿瘤坏死因子(TNF)-α含量增加,自然杀伤(NK)细胞活性减弱,铁蛋白(SF)、白细胞介素(IL)-2、γ干扰素(INF-γ)含量减少;与模型组比较,枸杞多糖组小鼠至给药第5天起体质量增加,给药10 d后瘤质量减少,AFP、CEA、TNF-α含量减少,NK细胞活性增强,SF、IL-2、INF-γ含量增加。结论:枸杞多糖能有效抑制H22肝癌模型小鼠癌细胞的生长,并能改善机体免疫水平。

OBJECTIVE： To study the effect of Lycium chinensis polysaccharides on tumor suppression and immune function of liver cancer model mice. METHODS ： Liver cancer model was induced by intraperitoneal injection of H22 tumor cell. 40 KM mice were randomly divided into normal control group （constant volume of normal saline）, model group （constant volume of normal saline）, cyclophosphamide group （i.p., 20 mg/kg）, and L. chinensis polysaccharides group （5 mg/kg）. They were given relevant medicine once a day for consecutive 10 days. The body weight, inhibition rate, serum tumor markers and inflammatory cytokines levels of each group were determined. RESULTS： Compared with normal control group, the body weight of mice was decreased in model group since fifth day of medication; tumor weight, the levels of AFP and CEA, TNF-α content were increased while the levels of SF, NK activity, IL-2 and INF-γ were decreased. Compared with model group, the body weight of mice was increased in L. chinensis polysaccharides group since fifth day of medication; tumor weight, the levels of AFP and CEA, TNF-α content were decreased while the levels of SF, NK activity, IL-2 and INF-γ were increased. CONCLUSIONS：L. chinensis polysaccharides can inhibit the growth of cancer cells in mice with H22 liver cancer, and can improve the body＇s immune level.