高效液相色谱法同时测定马铃薯及其土壤中的霜脲氰和嘧菌酯

Simultaneous Determination of Cymoxanil and Azoxystrobin in Potato and Soil by High Performance Liquid Chromatography

[目的]建立一种高效液相色谱分析方法,同时测定马铃薯及其土壤中的霜脲氰和嘧菌酯。[方法]样品用乙腈提取,CARB-NH2固相萃取柱净化后,以甲醇-水（体积比60：40）为流动相,流速为0.8 mL/min,使用BDS-C18色谱柱和紫外检测器,在254nm检测波长下,对霜脲氰和嘧菌酯进行分离检测。[结果]霜脲氰和嘧菌酯在0.05-2.0mg/L范围内线性良好,相关系数分别为0.999 8和0.999 9。在0.05-1.0 mg/kg添加水平下,霜脲氰的平均回收率为84.7%-94.2%,变异系数为1.0%-9.2%;嘧菌酯的平均回收率为94.0%-100.5%,变异系数为0.9%-6.9%。方法的最低检测质量浓度为0.125 mg/L。[结论]该方法简单、快捷、准确度和精密度高,适用于马铃薯及其土壤中霜脲氰和嘧菌酯的检测,也为霜脲氰和嘧菌酯在食品以及其他农产品中的监测提供参考。

[Aims] A high performance liquid chromatographic（HPLC） method was developed for the quantitative analysis of cymoxanil and azoxystrobin in potato and soil. [Methods] The samples were extracted with acetonitrile and purified by CARB-NH2solid-phase extraction column. The separation and determination were conducted by HPLC with BDS Hypersil C18 column and UV detector at 254 nm, and CH3OH-H2O（60：40, byvol）was utilized as the mobile phase at a flow rate of 0.8 mL/min. [Results] The method yielded a linear calibration curve in the concentration that range from 0.05 to 2.0 mg/L for cymoxanil and azoxystrobin and the correlation coefficients were 0.999 8 and 0.999 9,respectively. When the addition levels were 0.05-1.0 mg/kg, the average recoveries of cymoxanil and azoxystrobin were84.7-94.2% and 94.0-100.5% with the variation coefficients of 1.0-9.2% and 0.9-6.9%, respectively. In addition, the minimum detectable concentration of this method was 0.125 mg/L. [Conclusions] This method has advantages of simple, rapid, high accuracy and precision, therefore, it can be used for the determination of cymoxanil and azoxystrobin in potato and soil, it also provides a reference for analysis of cymoxanil and azoxystrobin in the food and other agricultural products.