血尿对细胞学和荧光原位杂交检测膀胱肿瘤结果的影响

Effect of hematuria on the performance of cytology and fluorescence in situ hybridization in the detection of bladder carcinoma

目的:探讨血尿对细胞学和荧光原位杂交(fluorescence in situ hybridization,FISH)检测膀胱肿瘤结果的影响。方法:2009年1月至2013年6月,164例可疑膀胱癌的患者均接受尿脱落细胞学、FISH、上尿路影像学检查和膀胱镜检等检查,对可疑病变取活检或(和)切除术。比较血尿对细胞学和FISH假阳性率和假阴性率的影响。结果:血尿和无血尿患者细胞学假阳性率分别为14.3%和20.9%(χ2=0.645,P=0.422),假阴性率分别为21.4%和39.1%(χ2=2.618,P=0.106);FISH假阳性率分别为19.0%和20.9%(χ2=0.047,P=0.828),假阴性率分别为30.3%和30.4%(χ2=0.002,P=0.967),均无统计学差异。结论:血尿不会影响细胞学和FISH检测膀胱肿瘤的结果。

Objective：To access the influence of hematuria on the performance of urine cytology（CYT）and fluorescence in situ hybridization（FISH）in the detection of bladder carcinoma（BC）. Methods：From January 2009 to June 2013,a total of 164 patients suspicious of BC underwent tests of CYT and FISH followed by upper urinary tract imaging and cystoscopy. Transurethral biopsy and/or resection were performed on suspicious lesions. The hematuria was determined by urine microscopy. Effect of hematuria on false positive and negative rates of patients with and without BC was compared. Results：For patients with hematuria,false positive rates of CYT were 14.3% and 20.9%（χ^2=0.645,P=0.422）and false negative rates of CYT were 21.4% and 39.1%（χ^2=2.618,P=0.106）;false positive rates of FISH were 19.0% and 20.9%（χ^2=0.047,P=0.828） and false negative rates of FISH were 30.3% and 30.4%（χ^2=0.002,P=0.967）all without statistical difference. Conclusion：Hematuria does not influence the performance of CYT and FISH.