过表达MiR-494抑制脑胶质瘤细胞U87的增殖

Overexpressed miR-494 inhibits the proliferation of U87 glioma cells

目的观察微小RNA-494(miR-494)对脑胶质瘤细胞U87增殖能力的影响。方法通过实时荧光定量聚合酶链式反应(qRT-PCR)法检测5例瘤旁组织和14例胶质瘤样本中miR-494的表达水平,并且检测其在6种胶质瘤细胞系中的表达;将100 nmol/L miR-494 mimics瞬时转染至脑胶质瘤U87细胞,qRT-PCR检测miR-494表达情况;采用四唑盐比色法(MTT)检测U87细胞增殖情况;流式细胞术(FCM)检测对U87细胞周期的影响。结果与瘤旁脑组织相比,miR-494在胶质瘤中呈现低表达;U87细胞转染48 h后,miR-494 mimics组miR-494表达为对照组的357倍(P<0.05);与对照组相比,miR-494 mimics转染后抑制了U87细胞的增殖能力(P<0.05);S期细胞显著减少,而G1期细胞则明显增多。结论 miR-494在胶质瘤中低表达,过表达miR-494 mimics有效抑制了U87细胞的增殖,调节细胞周期S期降低G1期增多,提示miR-494有望成为胶质瘤治疗的分子靶点。

Objective The role of miR-494 on the cell proliferation of human U87 glioma cell lines is discussed. Methods Quantitative real-time reverse transcription polymerase chain reaction（ qRT-PCR）was applied to detect the expression of miR-494 in 5 human normal brain tissues,14 glioma tissues and 6glioma cell lines; miR-494 was transiently transfected into U87 glioma cells（ 100 nmol / L）; Mosmann Tetrazolium Test（ MTT） assay was used to detect the cell proliferation; the cell cycle was measured by flow cytometry（ FCM）. Results miR-494 was low expressed in glioma tissues compared with normal samples.The expression of miR-494 was as 357 folds as the control（P 0. 05） after transient transfecting with miR-494 mimics. Decreased proliferation and G1-to-S cell cycle progression were detected in the miR-494 mimics transfected U87 glioma cells（ P 0. 05）. Conclusion The expression of miR-494 is aberrantly low in glioma tissues. Transient transfection of miR-494 mimics leads to decreased proliferation and G1-to-S cell cycle progression in U87 glioma cells,indicating that miR-494 might be a new molecular target for the treatment for glioma.