KiSS-1和MMP-9在胃癌及其癌前病变中的表达和临床病理意义

Clinicopathological significance and expression of KiSS-1 and MMP-9 in gastric carcinoma and the precancerous lesions

目的:检测KiSS-1与MMP-9在胃癌中的表达,分析两者与胃癌临床病理因素以及患者临床预后之间的关系,探讨KiSS-1和MMP-9在胃癌发生发展中的作用。方法:将162例胃癌及配对正常组织构建于组织芯片上,采用免疫组化两步法检测KiSS-1和MMP-9的表达。结果:胃癌组织中KiSS-1的阳性表达率(67.90%,110/162)显著低于正常黏膜(83.95%,136/162)、慢性萎缩性胃炎(90.48%,38/42)和肠上皮化生(83.87%,78/93)中的阳性率,P<0.05。胃癌组织(78.40%,127/162)、慢性萎缩性胃炎(52.38%,22/42)和肠上皮化生(68.82%,64/93)中MMP-9的阳性表达率显著高于正常黏膜(31.48%,51/162)。MMP-9在肠型胃癌(90.48%,38/42)中的阳性表达率显著高于弥漫型胃癌(74.11%,83/112)(P<0.05)。MMP-9表达强阳性组和其他组相比[(15.2±4.8)个月vs(35.0±2.9)个月],平均生存时间较短,差异有统计学意义(P=0.032)。结论:与正常胃黏膜相比,胃癌组织中存在着KiSS-1表达下调和MMP-9表达的上调,可能在胃癌发生发展过程中发挥着重要作用。此外,MMP-9的强阳性表达与不良预后有关。

Objective：To explore the relationship between KiSS-1 and MMP-9 expression in gastric carcinomas （GC）,to analyze the correlation between KiSS-1 and MMP-9 expression in gastric carcinoma and relations with clinicopathological features.Methods：All 162 cases of tumor specimens were constructed in tissue microarrays.KiSS-1 and MMP-9 expression was investigated by two-step immunohistochemical detection.Results：The positive rate of KiSS-1 expression was significantly lower in gastric carcinoma（GC） （67.90%,110/162） than in normal gastric （83.95 %,136/162）,chronic atrophic gastritis （CAG） （90.48 %,38/42） and intestinal metaplasia （IM） （83.87%,78/93） （P 〈 0.05）.The positive rate of MMP-9 expression in GC （78.40%,127/162）,CAG （52.38%,22/42） and IM（68.82%,64/93） was significantly higher than in normal gastric mucosa（31.48%,51/162）.Full-negative group of KiSS-1 expression （45.45%,10/22） in the Borrmann Ⅰ type and Borrmann Ⅱ type higher than the other groups（12.14,17/140）,the difference was statistically significant（P =0.001）.MMP-9 positive expression in intestinal type gastric cancer（90.48%,38/42） was significantly higher than diffuse-type gastric cancer（74.11%,83/112） （P 〈 0.05）.Strong positive group of MMP-9 （15.2 ± 4.8） months compared with other groups（35.0 ±2.9） months the average survival time was shorter,the difference was statistically significant （P =0.032,log-rank test）.Conclusion：Decreased expression of KiSS-1 and increased expression of MMP-9 exist in gastric carcinomas,and is related to certain clinicopathological parameters,suggesting they play important roles in gastric carcinogenesis.The strong positive expression of MMP-9 was associated with poor prognosis.