纯钛在仿真蛋白环境中腐蚀性能的电化学阻抗谱分析

Electrochemical impedance spectroscopic study of titanium in physiological environment with blood-serum protein

目的:在高仿真环境中,采用电化学阻抗法分析纯钛种植材料的腐蚀性能。方法 :以PBS液为对照组,细胞培养液为实验组,采用电化学分析仪测试钛在含血清蛋白体系中的电化学阻抗谱,利用EIS-Nyquist图、EIS-Bode图及等效电路的拟合分析,研究血清蛋白对钛的抗腐蚀性能的影响,采用SPSS11.0软件包对数据进行统计学分析。结果:纯钛材料在细胞培养液中的氧化膜阻抗值(24.8±3.9)×105Ω显著大于在PBS液中的阻抗值(6.7±0.8)×105Ω(P<0.05)。纯钛在PBS中低频区的相位角约为55°,而在细胞培养液中低频区的相位角约为75°。结论:机体环境中的血清蛋白能够影响钛金属在体液中的腐蚀性能。

PURPOSE： Electrochemical impedance spectroscopy （EIS） was employed to study the corrosion behaviour of titanium in dummy environment with blood-serum protein. METHODS： EIS of titanium in PBS and cell cuhure fluid was acquired by advanced electrochemical system. The characteristic of EIS-Nyquist and EIS-Bode were investigated and the equivalent circuit was used to analyze the EIS results. The data was analyzed using SPSS 11.0 software package. RESULTS： The corrosion resistance of the titanium in cell culture fluid was significantly higher than that in PBS （P〈 0.05）. The phase angle of TiO2-based passive film at low frequency decreased to 55°when titanium was in PBS, but 75° when in cell culture fluid. CONCLUSIONS： The blood-serum protein plays an important role in the corrosion behaviour of titanium in physiological environment. Supported by Research Fund of Science and Technology Committee of Shanghai Municipality （08DZ2271100）.