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2ME2对脊髓损伤大鼠运动功能及Tau蛋白磷酸化的影响 被引量：5

Effect of 2-methoxyestradiol on phosphorylation of Tau protein and motor function of rats after spinal cord injury
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摘要 目的探讨2-甲氧基雌二醇（2ME2）对脊髓损伤大鼠运动功能及Tau蛋白磷酸化的影响。方法成年雄性SD大鼠72只按随机数字表法分为假手术组、脊髓损伤组和2ME2治疗组，每组24只。后2组大鼠采用改良的Allen’s法制作脊髓损伤大鼠模型。造模后1d 2ME2治疗组大鼠腹腔注射2ME2（24mg/kg），连续7d。脊髓损伤组大鼠注射等剂量生理盐水，假手术组不予干预。造模后1、3、7、14、21、28d应用BBB评分法对各组大鼠的后肢功能进行评分。造模后7d应用免疫荧光染色和Western blotting检测损伤脊髓磷酸化Tau蛋白（p-Tau）的表达。结果脊髓损伤组和2ME2治疗组大鼠脊髓损伤后1-28d随着时间的延长BBB评分升高，差异有统计学意义（P〈0．05）。损伤后7、14、21、28d2ME2治疗组大鼠BBB评分明显高于脊髓损伤组．差异有统计学意义（P〈0．05）；与假手术组比较，脊髓损伤组和2ME2治疗组大鼠脊髓p-Tau阳性细胞数、蛋白表达均增加，且2ME2治疗组p-Tau阳性细胞数、蛋白表达（19．05±1．34、0．283±0．0941明显少于脊髓损伤组（10．36±1．28、0．607±0．105），差异均有统计学意义（P〈0．05）。结论2ME2可改善脊髓损伤大鼠的运动功能，具有一定的神经保护作用，其可能通过降低Tau蛋白磷酸化水平来实现。 Objective To investigate the effect of 2-methoxyestradiol （2ME2） on phosphorylation of Tau protein （p-Tau） and mortor function of rats after spinal cord injury （SCI）. Methods A total of 72 adult male Sprague-Dawley rats were randomly divided into 3 groups： sham-operated group （n=24）, SCI group （n=24） and SCI＋2ME2 treated group （n=24）; models of SCI were created by modified Alien＇s method; one d after SCI, rats in the SCI＋2ME2 treated group were given intraperitoneal injection of 2ME2 （24 mg/kg） for seven d, and rats in the SCI group were given the same volume of normal saline. Basso, Beatti, Bresnahan （BBB） scale was performed to evaluate the hindlimb function one, three, seven, 14, 21 and 28 d after SCI; the expression changes of p-Tau （Ser 262） seven d after SCI were observed by immunofluorescence and Western blotting. Results BBB scale scores in the SCI group and SCI＋2ME2 group were significantly increased following the prolonging of injury times （I-28 d of injury, P〈0.05）; on the 7^th, 14^th, 21^st and 284 d of injury, the scores in the SCI＋2ME2 group were significantly higher than those in the SCI group （P〈0.05）. The p-Tau positive cells in the spinal cord and the p-Tau （Ser 262） expression in the SCI group and SCI＋2ME2 group were significantly increased as compared with those in the sham-operated group （P〈0.05）, and those in the SCI＋2ME2 group （19.05±1.34 and 0.283±0.094） were obviously lower as compared with those in the SCI group （10.36±1,28 and 0.607±0.105, P〈0.05）, Conclusion 2ME2 offers protection in rats with SCI by reducing the level of tau phosphorylation.
作者 张鸿日 彭静华 张茂营 周洪龙 徐如祥
机构地区 南方医科大学珠江医院神经外科广东神经外科研究所广东省脑功能修复与再生重点实验室 河南科技大学第一附属医院神经内科 北京军区总医院附属八一脑科医院
出处 《中华神经医学杂志》 CAS CSCD 北大核心 2014年第11期1127-1130,共4页 Chinese Journal of Neuromedicine
基金 国家临床重点建设专科资助项目 广东省神经外科临床中心资助项目
关键词 脊髓损伤 2.甲氧基雌二醇 TAU蛋白 磷酸化 Spinal cord injury 2-methoxyestradiol Tau protein Phosphorylation
分类号 R651.2 [医药卫生—外科学]
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参考文献9

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共引文献4

同被引文献49

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二级引证文献8

中华神经医学杂志
2014年 第11期

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