知母盐制前后对α-葡萄糖苷酶抑制作用比较

Comparion on the Inhibitory Activities of α- glucosidase from Anemarrhenae Rhizoma before and after Processed with Salt-water

目的 比较知母和盐知母提取物对α-葡萄糖苷酶的抑制作用,从体外方面探讨知母不同炮制品的降血糖作用.方法 以对-硝基苯基-α-D-吡喃葡萄糖苷（PNPG）为底物,采用HPLC法分析水解产物中的对-硝基酚（PNP）浓度,比较知母、盐知母5种不同浓度的水煎液对α-葡萄糖苷酶的抑制作用.结果 在低浓度下,各组均表现出较弱的抑制作用;在2.6 ～65mg/ml浓度下,知母、盐知母水煎液均表现出一定的抑制作用,盐知母抑制作用强于知母,与浓度呈正相关,且优于阳性对照阿卡波糖.结论 知母和盐知母均能抑制α-葡萄糖苷酶的活性,盐制后作用增强.

Objective To compare the inhibitory effect of anemarrhenae rhizoma and salt anemarrhenae rhizoma on α - glucosidase, thus to investigate the hypoglycemic effect of different processed products of anemarrhenae rhizoma in vitro. Methods PNPG bing taken as the substrate, the inhibitory activity against α - glucosidase of 5 different concentrations extracts were analyzed with the contents of PNP in hydrolyzate by HPLC method. Results All groups showed weak inhibition at low concentrations. In 2.6 -65mg/ml concentrations, anemarrhenae rhizoma and salt anemarrhenae rhizoma groups showed a certain inhibition, which was positively correlated with the concentration. The sah group was stronger than anemarrhenae rhizoma group, and better than the positive control group acarbose. Conclusion Both anemarrhenae rhizoma and salt anemarrhenae rhizoma can inhabite α -glucosidase activity and enhance hypoglycemic effect significantly after processing.