龙丹生血颗粒对免疫性血小板减少性紫癜模型小鼠外周血象与骨髓巨核细胞以及血清PAIgG水平的影响

Effect of Long Dan Sheng Xue Granule on the Peripheral Hemogram,Bone Marrow Megakaryocytes and Serum PAIgG Level in Model Mice with Immune Thrombocytopenic Purpura

目的观察龙丹生血颗粒对ITP模型小鼠骨髓巨核细胞和血清PAIgG的影响。方法 70只Balb/c小鼠随机分为对照、模型、升血小板胶囊、泼尼松、龙丹生血颗粒大、中、小剂量7组,除正常组外,其余各组隔日1次腹腔注射豚鼠抗小鼠血小板血清(GP-APS)建立ITP小鼠模型,于造模第8天开始给药,各组均按0.2ml/(10g·d)体积灌胃。其中,正常组、模型组灌服生理盐水,升血小板胶囊组灌胃升血小板胶囊内容物混悬液1.125g/kg,泼尼松按0.0113mg/(10g·d)灌胃,龙丹生血颗粒大、中、小剂量组分别按13.75g生药/千克、6.88g生药/千克、3.44g生药/千克灌胃。连续8天后眼球取血分离血清,进行血液学、血清PAIgG检测,并观察小鼠骨髓巨核细胞形态及分类。结果与正常组相比,模型组小鼠PLT、WBC、Hb降低,血清PAIgG明显升高,骨髓产板巨核细胞数减低。与模型组相比,龙丹生血颗粒各剂量组小鼠外周血PLT、WBC、Hb数值均有所恢复。大剂量组产板巨核细胞明显增加,PAIgG有所降低。结论龙丹生血颗粒通过降低血清PAIgG水平、促进骨髓巨核细胞分化而提升ITP模型动物外周血小板数值。

Objective To observe the effect of Long Dan Sheng Xue granule on the peripheral hemogram,bone marrow megakaryocytes and serum PAIgG Levelin model mice with immune thrombocytopenic purpura（ITP）.Methods Seventy Balb/c mice were randomly divided into seven groups as control,model,Sheng Xue Xiao Ban capsule（ascending platelet capsule）,prednisone,Long Dan Sheng Xue granule large,middle,small doses group.Except the control group,mice in each group was intraperitoneal injected guinea pig antimouse platelet serum （GP-APS） once every other day to establish ITP mice model.From the eighth day of model making,mice in each group was administered O.2ml/ （10g · d） volume of drugs by intragastric administration.Among them,mice in the control group and model group were given normal saline; Sheng Xue Xiao Ban capsule（ascending platelet capsule） group were given Sheng Xue Xiao Ban capsule contents suspension by 1.125g/kg,Prednisone group was given prednisone by 0.0113mg/ （10g · d）,Long Dan Sheng Xue granule large,middle and small dose groups were administered by 13.75g crude drug/kg,6.88g crude drug/kg and 3.44g crude drug/kg respectively.After eight days of continuous administration,the eyeball was taken for blood and the serum was separated,the hemogram and serum PAIgG level were detected and the bone marrow megakaryocyte morphology and classification were observed.Results Compared with the control group,the PLT,WBC and HGB of model group decreased,the level of serum PAIgG increased significantly,the number of platelet-forming megakaryocytes reduced.Compared with the model group,the PLT,WBC and HGB of each dose group of Long Dan Sheng Xue granule were all recovered to some extent,while for the large dose group,the number of platelet-forming megakaryocytes increased significantly and the PAIgG level was lowered somewhat.Conclusion Long Dan Sheng Xue granule can increase peripheral platelet counts of model animals with ITP by reducing serum PAIgG level and then promoting bone marrow megakaryocyte differentiation.