小麦TaMAPK2基因的克隆及表达分析

Cloning and Expression Analysis of TaMAPK2 Gene in Wheat

该研究从小麦中克隆了1个MAPK基因TaMAPK2。序列分析表明,TaMAPK2基因的ORF为1 110bp,编码369个氨基酸。序列比对分析表明,该基因所编码的蛋白与粗山羊草、水稻、谷子等MAPK蛋白具有较高的一致性,分别为99%、94%、94%。进化树分析表明,TaMAPK2与水稻OsMAPK2的亲缘关系最近。实时荧光定量PCR分析表明,该基因的表达显著受渗透胁迫、低温胁迫、高盐胁迫、乙烯和双氧水诱导,受ABA抑制。研究表明,TaMAPK2可能参与非生物逆境胁迫及相关信号分子应答。

MAPK protein kinase plays an important role in the response of abiotic stresses. In the present study,we isolated a MAPK gene designated TaMAPK2 from wheat. TaMAPK20RF is 1 110 bp,which encodes 369 amino acids. Comparison of amino acid sequences indicated that TaMAPK2 shared high simi larity with MAPKs from other species,such as Aegilops tauschii （99%） ,Oryza sativa （94%） and Setaria italica （94%）. Phylogenetic analysis showed that TaMAPK2 is closely related with OsMAPK2. Real-time quantitative polymerase chain reaction （QPCR） assay revealed that TaMAPK2 expression is induced after osmotic stress, low temperature, salt stress, ethylene and H202 treatments and inhibited after ABA treatment. These results suggested that TaMAPK2 participates in the response of abiotic stresses and related signaling molecules.