铜锌复合污染对铜富集植物大聚藻抗氧化酶活性的影响

Effect of Cu and Zn Compound Pollution on Antioxidant Enzyme Activity of Cu-enrichment Plant Myriophyllum aquaticum

以前期筛选的铜富集植物大聚藻为材料,采用两因素随机区组试验设计,通过盆栽试验研究了不同浓度铜锌复合污染对大聚藻抗氧化酶活性的影响,以揭示铜富集植物大聚藻对重金属的耐性机理,为芦溪河及其它类似污染河流的生态恢复与植被重建提供参考依据。结果表明:(1)铜锌复合污染条件下,大聚藻生物量都表现出低促高抑现象。(2)铜锌复合污染时,大聚藻MDA含量随铜锌浓度升高表现出先升高后降低的变化。(3)铜锌复合污染对大聚藻抗氧化酶系统活性均有不同程度的影响,低浓度铜锌复合污染对SOD(超氧化物歧化酶)、POD(过氧化物酶)和CAT(过氧化氢酶)有促进作用,而随浓度的升高则表现出不同的规律。研究发现,铜锌胁迫下,大聚藻细胞应急防御系统被启动,SOD、POD和CAT发挥作用,体内过量自由基及时被清除,使大聚藻能够保持高的耐性。

The pre-screeing ,Cu-randomized block experiment design, aquaticum ment material. Through two factor M. aquaticurn under different concentrations of Cu and Zn contaminated soil had been conducted by pot experiment. The results indicated thatUnder different concentrations of Cu and Zn compound pollution, the biomass of M. aquaticum was promoted by the low concentration pollution and inhibited by the highconcentration poliution. （2）Under different concentrations of Cu and Zn compound pollution, the MDA content of M. aquaticura with the increase of Cu-Zn concentration showed after the first rise to reduce. （3） Different concentrations of Cu and Zn compound pollution played different roles on the activity of antioxi- dant enzyme system of M. aquaticum. The low concentration of Cu and Zn compound pollution improved the activities of SOD,POD and CAT,while the increased concentration of Cu and Zn compound pollution showed different effects on the activities of SOD, POD and CAT. Based on the data reported, under the pressure of the combination of Cu and Zn,M. aquaticura cell activate the emergency defense system, then SOD,POD and CAT start to work to removal of excess free radicals in the body in time,so that maintain the high tolerance of M aquaticum material of ecological restoration and reconstruction of vegetation in Luxi river and other similar rivers contaminated by Cu and Zn.