12种不同产地铁皮石斛指纹图谱研究及重金属元素含量测定

Fingerprints and Heavy Metal Contents of Dendrobium officinale Produced from 12 Areas

以12种不同产地铁皮石斛新鲜茎段为材料,以Ultimate XB C18 （4.6 mmx250 mm,5μm）乙腈0.1％磷酸溶液为流动相进行梯度洗脱,流速1.0 mL·min-1,检测波长270 nm,柱温30℃.通过中药色谱指纹图谱相似度评价系统（国家药典委员会）进行相似度分析,并建立12个不同产地铁皮石斛指纹图谱.经微波消解处理材料后,采用ICP-AES原子吸收光谱法测定不同产地铁皮石斛新鲜茎段中重金属铜、镉、铅、汞的含量,分析不同产地铁皮石斛质量,为建立铁皮石斛质量标准提供理论依据.结果表明：（1）不同产地铁皮石斛指纹图谱共有23个特征峰,相似度为0.918～0.987,其中产自安徽大别山、云南文山的铁皮石斛相似度分别为0.918、0.935,相比其他产地具有一定的差异性.（2）通过聚类分析将12种不同产地铁皮石斛分为4类：安徽霍山、江苏南京、云南石屏、云南玉溪、云南思茅为一类,云南文山、浙江仙居为一类,云南红河、广西天峨、安徽大别山、福建宁化为一类,浙江天台单独为一类.（3）产自云南玉溪的铁皮石斛茎段中铜含量超标26.25％,产自福建宁化和江苏南京的镉含量分别超标27.33％和122.30％,其它产地铜、镉、铅、汞含量在国家现行安全值以内.

With different origins of Dendrobium officinale fresh stem produced from 12 different areas seg- ments as material,we performed gradient elution,with Ultimate XB C18 （4.6 mm）〈 250 mm,5 μm） acetonitrile-0, l% phosphoric acid solution as mobile phase gradient elution,velocity of 1.0 mL ·min-1 ,detection wavelength of 270 nm, column temperature 30 ~C. Through Chinese medicine chromatographic fingerprint similarity evaluation system （national pharmacopoeia committee） similarity analysis and establish the 12 different areas fingerprint,at the same time by microwave digestion processing materials, using ICP-AES atomic absorption spectrometry determination of different origin of cadmium, copper, lead and mercury con- tents, so as to compare the different origin of quality, to provide theoretical basis for establishing quality standard. The results show： （1）A total of 23 different producing area features was established. The similarity of different origin of D. officinale was 0. 918--0. 987 ,which shows differences,but there was no difference in the chemical composition and chemical contents; （2）Through clustering analysis,D, officinale of12 different regions can be divided into four categories：Anhui Huoshan,Jiangsu Nanjing,Yunnan Shiping, Yunnan Yuxi, Yunnan Simao, as a category, Yunnan Wenshan, Zhejiang Xianju as a category, Yunnan Honghe,Guangxi Tian’e, Dabie Mountain in Anhui and Fujian Ninghua as a class, Zhejiang Tiantai for a class;（3） Including the copper contents from Yuxi of Yunnan, cadmium contents from Fujian Ninghua,Jian- gsu Nanjing,exceed 26.25%, 27.33% and 122.30%, respectively. Other orgin of copper, cadmium, lead, mercury levels in countries. Our aim is to provide theoretical basis for D. officinale medicinal materials quality control.