逍遥散对急性肝损伤小鼠的肝脏保护作用

Liver protective effects of Xiaoyaosan in acute liver injury in mice

目的：观察逍遥散对四氯化碳（CCl4）致小鼠急性肝损伤的肝脏保护作用。方法将30只小鼠按照随机数字表法分为空白对照组、模型组、逍遥散组，逍遥散组灌胃逍遥散，其余2组灌胃等量蒸馏水。给药后7 d，腹腔注射CCl4花生油溶液诱导小鼠急性肝损伤，16 h后取材，检测小鼠血清丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、肝组织丙二醛（MDA）、超氧化物歧化酶（SOD）浓度，并取肝组织做病理学观察。结果模型组小鼠 ALT、AST、MDA 水平明显升高，SOD 水平明显降低[分别为（136.46±15.75）U/L、（145.37±16.39）U/L、（17.48±3.45）nmol/mg、（261.60±20.29）U/mg]，与空白对照组比较[分别为（22.96±6.23）U/L、（31.89±7.26）U/L、（4.22±1.08） nmol /mg、（336.73±25.34）U/mg]，差异有统计学意义（P＜0.01）。逍遥散组ALT、AST、MDA水平明显降低，SOD水平升高[分别为（89.38±6.96）U/L、（119.04±20.44）U/L、（10.30±2.22）nmol/mg、（304.77±31.71）U/mg],与模型组比较差异有统计学意义（P＜0.01或0.05）。结论逍遥散对CCl4致小鼠急性肝损伤有保护作用。

Objective To investigate the liver protective effects of Xiaoyaosan in carbon tetrachloride-induced acute liver injury in mice.Methods Thirty mice were randomly divided into a control group, a model group, and aXiaoyaosan group, with 10 mice in each group. The mice in theXiaoyaosan group were intragastrically administrated withXiaoyaosan, the mice in the remaining two groups were fed with an equal volume of distilled water. After 7 days, acute liver injury were inducedvia intraperitoneal injection of carbon tetrachloride peanut oil solution. The serum levels of alanine transaminase（ALT）, aspartate transaminase （AST）, malondialdehyde（MDA）and superoxide dismutase（SOD）were measured, and pathological changes of liver tissue was tested after 16 hours.Results The serum levels of ALT（136.46±15.75 U/Lvs. 22.96±6.23 U/L）, AST（145.37±16.39 U/Lvs. 31.89±7.26 U/L）, and MDA level in the liver tissue（17.48±3.45 nmol/mgvs. 4.22±1.08 nmol/mg）in the model group were significantly higher than those in the control group（allP〈0.01）, SOD level in the liver tissue significantly lower than that in the control group（261.60±20.29 U/mgvs. 336.73±25.34 U/mg,P〈0.01）. The serum levels of ALT（89.38±6.96 U/L,P〈0.01）, AST（119.04±20.44 U/L, P〈0.05）, MDA level（10.30±2.22 nmol/mg,P〈0.01） in the liver tissue in theXiaoyaosan group levels were significantly lower than those in the model group, and SOD level（304.77±31.71 U/mg,P〈0.01） in the liver tissue were significantly higher than that in the model group.ConclusionXiaoyaosan has liver protective effects in carbon tetrachloride-induced acute liver injury in mice.