苦参碱和氧化苦参碱对人肝癌细胞株 SMMC-7721凋亡的影响

Effects of matrine and oxymatrine on apoptosis in human hepatocarcinoma SMMC-7721

目的：探讨苦参碱与氧化苦参碱对人肝癌SMMC-7721细胞凋亡的影响。方法采用MTT法检测SMMC-7721细胞增殖，并采用膜联蛋白V-异硫氰酸荧光素/碘化丙啶双染法检测SMMC-7721细胞凋亡。结果苦参碱与氧化苦参碱浓度为0.50～2.00 mg/ml时，细胞增殖抑制率均逐渐上升，呈药物浓度和作用时间的依赖性；同样浓度下（1.00 mg/ml），苦参碱增殖抑制作用强于氧化苦参碱[24 h、48 h和72 h时苦参碱组分别为（42.39±0.04）%、（51.69±0.03）%、（78.98±0.05）%，氧化苦参碱组分别为（21.36±0.02）%、（36.16±0.02）%、（61.24±0.13）%；P均＜0.05]。苦参碱与氧化苦参碱浓度为0.25、0.50、1.00 mg/ml时SMMC-7721细胞凋亡率显著增高；同样时间点（48 h），苦参碱对细胞凋亡的诱导强于氧化苦参碱[0.25、0.50、1.00 mg/ml时，苦参碱组凋亡率分别为（4.08±0.20）%、（4.32±0.19）%、（9.93±0.18）%，氧化苦参碱组分别为（2.20±0.18）%、（3.08±0.26）%、（9.01±0.20）%；P均＜0.05]。结论苦参碱和氧化苦参碱可抑制人肝癌SMMC-7721细胞增殖，促进其凋亡。

Objective To explore the effects of matrine and oxymatrine on apoptosis in human hepatocarcinoma SMMC-7721 cells.Methods The MTT assay and double staining of annexin V-fluorescein isothiocyanate（annexin V-FITC）/propidium iodide（propidium iodide, PI）were used to detect proliferation and apoptosis of SMMC-7721 cells, respectively.Results When the concentrations of matrine and oxymatrine were 0.50 mg/ml, 1.00 mg/ml and 2.00 mg/ml, the proliferation inhibition rates in SMMC-7721 cells was gradually increased in a dose- and time-dependent manner,and the inhibition of matrine on proliferation were greater than that of oxymatrine in the same concentration（1.00 mg/ml）（24 h：42.39%±0.04%vs. 21.36%±0.02%;48 h： 51.69%±0.03%vs. 36.16%±0.02%;72 h： 78.98%±0.05%vs. 61.24%±0.13%;allP〈0.05）. When the concentrations of matrine and oxymatrine were 0.25 mg/ml, 0.50 mg/ml and 1.00 mg/ml, the apoptosis rates of SMMC-7721 cells were significantly increased;and induction of matrine in apoptosis in SMMC-7721 cells was greater than that of oxymatrine at the same time point（48 h）（apoptosis rates in 0.25 mg/ml, 4.08%±0.20%vs. 2.20%±0.18%;0.50 mg/ml： 4.32%±0.19%vs. 3.08%±0.26%;1.00 mg/ml： 9.93%±0.18%vs. 9.01%±0.20%;allP〈0.05）.Conclusion Matrine and oxymatrine can inhibit proliferation and promote apoptosis human hepatocarcinoma SMMC-7721 cells.