摘要
目的 观察大鼠肢体缺血再灌注后肺组织P-选择素表达的变化及内皮素A受体阻断剂BQ123对其影响.方法 雄性Wistar大鼠随机分为3组（n=10）：对照组、再灌注组和BQ123处理组.缺血4h再灌注4h后采用ELISA方法定量检测肺P-选择素浓度,测定各组PaO2、BALF蛋白含量,测定肺组织髓过氧化物酶、内皮素1的含量,观察肺组织病理变化.结果 与再灌注组比较,BQ123处理组肺组织P-选择素含量明显降低,PaO2升高,BALF中总蛋白、肺组织髓过氧化物酶、内皮素1明显降低（P值均＜0.01）,肺组织形态损伤减轻.结论 BQ123抑制了大鼠肢体缺血再灌注后肺组织P-选择素的表达,减轻肺损伤.
Objective To observe the expression of P-selectin in lung tissue following limb ischemia reperfusion in rats and investigate the role of endothelin-A receptor antagonist BQ123 in it.Methods Male Wistar rats were randomly divided into three groups （n =10）：control group,LIR group,and BQ123 treated group.ELISA method was used to detect the concentration of P-selectin.PaO2,the total protein content in bronchoalveolar lavage fluid,the levels of myeloperoxide and endothelin-1 in lung tissue were measured.The pathological changes of lung tissue were observed.Results Compared with LIR group,the level of P-selection in lung tissue was decreased,PaO2 was increased,the protein content in bronchoalveolar lavage fluid,the contents of myeloperoxide and endothelin-1 in lung tissue were lower in BQ123 group （all P ＜0.01）.Histopathological examination showed that the degree of lung injury was alleviated in BQ123 group.Conclusions BQ123 inhibits the formation of P-selection to have protective effects on lung tissue after limb ischemia reperfusion in rats.
出处
《国际呼吸杂志》
2014年第20期1551-1553,共3页
International Journal of Respiration
基金
河北省科学技术研究与发展计划项目（12277793）
唐山市科技局科研基金资助课题（12140209A-27）
