HPLC法同时测定川芎药材中阿魏酸、咖啡酸的含量

Determination of Ferulic acid, and Caffeic acid in Rhizoma Chuanxiong. by HPLC

目的：建立 HPLC 法同时测定川芎药材中阿魏酸和咖啡酸含量的方法。方法采用 Kromasil -ODS （250mm×4.6mm，5μm）色谱柱，流动相为甲醇-0.5%醋酸水（35：65）；流速：1.0mL/min；检测波长：323nm。结果阿魏酸和咖啡酸的线性范围分别为：13.62～257.4mg/mL（r=0.9996）和29.40～515.7mg/mL （r=0.9995）；平均回收率（n=6）分别为阿魏酸96.6%（RSD=0.8%）和咖啡酸95.6%（RSD=0.5%）。结论该法操作简单，结果准确，重现性好，为全面评价不同产地川芎药材的质量提供方法。

Objective To develop an HPLC method for Ferulic acid, Caffeic acid in Rhizoma Chuanxiong. Methods Kromasil -ODS （250mm×4.6mm, 5μm） column was used to separate the sample at the column temperature of 35 ℃ . Methanol was used with a mobile phase of methanol -0.5% aetic acid （35：65, v/v） at a flow rate of 1.0mL/min. The detection wavelengths was set at 323nm. Results The calibration curve was linear over the concentration of 13.62-257.4μg/mL for ferulic acid （r=0.9995）. 29.40-515.7μg/mL for Caffeic acid （r=0.9996）. The average recovery of Ferulic acid and Caffeic acid were 96.6%, 95.6%, and the RSD were 0.8%, 0.5%, respectively. Conclusion This method is smiple and reproducible, which can be used for the quality control of Rhizoma Chuanxiong.