摘要
To obtain water-insoluble silk fibroin （SF） materials, polyethylene glycol diglycidyl ether （PEG-DE） was selected as a crosslinking agent to prepare SF films （blends）. The reaction conditions were optimized for the crosslinking of the SF molecules. The hot water stability of the blends was measured using BCA protein assay and gravimetric analysis. The molecular conformation and crystalline structure of the blends were analyzed by FTIR and XRD, respectively. When the mass ratio of SF：PEG-DE was 1.0：0.8, the hot water loss rate of the SF blends was minimized. PEG-DE could induce SF molecules to form fl-sheets during the gel reaction process, resulting in improved crystallinity and hot water dissolved resistance of the blend films. In order to demonstrate the eytotoxicity of the chemical reagents used to crosslink SF, L929 cells were seeded on the blend film （SF：PEG-DE = 1：1） and cultured for 3 days. Cells of L929 readily adhered and spread in the fusiform on the blend film resulting in high cell viability. The extracted liquid from the SF porous film did not inhibit cell proliferation, as estimated by the MTT assay.
To obtain water-insoluble silk fibroin （SF） materials, polyethylene glycol diglycidyl ether （PEG-DE） was selected as a crosslinking agent to prepare SF films （blends）. The reaction conditions were optimized for the crosslinking of the SF molecules. The hot water stability of the blends was measured using BCA protein assay and gravimetric analysis. The molecular conformation and crystalline structure of the blends were analyzed by FTIR and XRD, respectively. When the mass ratio of SF：PEG-DE was 1.0：0.8, the hot water loss rate of the SF blends was minimized. PEG-DE could induce SF molecules to form fl-sheets during the gel reaction process, resulting in improved crystallinity and hot water dissolved resistance of the blend films. In order to demonstrate the eytotoxicity of the chemical reagents used to crosslink SF, L929 cells were seeded on the blend film （SF：PEG-DE = 1：1） and cultured for 3 days. Cells of L929 readily adhered and spread in the fusiform on the blend film resulting in high cell viability. The extracted liquid from the SF porous film did not inhibit cell proliferation, as estimated by the MTT assay.
基金
Funded by National Natural Science Foundation of China(Nos.51173125 and 51473108)
Natural Science Foundation of Jiangsu Province of China(Nos.BK2012633 and BK2041210)
College Natural Science Research Project of Jiangsu Province of China(No.12KJA43004)
the Science and Technology Development Foundation of Suzhou of China(Nos.SYG201001 and SS201341)
Priority Academic Program Development of Jiangsu Higher Education Institutions[PAPD]
