ApoAI对原代培养人Müller细胞生长的影响

Effects of ApoAI on the growth of cultured human retinal Müller cells

目的:研究ApoAI对原代培养人Müller细胞生长的影响。方法:原代培养人的视网膜Müller细胞后用不同浓度的ApoAI刺激Müller细胞24 h,采用流式细胞术检测ApoAI作用24 h后Müller细胞的细胞周期,比较各组细胞的细胞周期;采用流式细胞术和荧光照相法检测各组Müller细胞细胞凋亡水平,采用单因素方差分析方法比较各组间不同细胞周期间不同细胞数的变化以及各组细胞的凋亡数。结果与结论:流式细胞术结果显示,ApoAI可以抑制人Müller细胞的增殖,且随ApoAI浓度的增加抑制作用增强(P<0.05);ApoAI对细胞的凋亡无明显作用(P>0.05)。

Objective： To investigate effects of ApoAI on the growth of cultured human retinal M uller cells. Methods：In vitro,human retinal Muller cells were successfully cultured from the remaining part of the eye after corneal transplantation .With different concentrations of ApoAI stimulated , the cycles and the levels of apoptosis of Muller cells were detected by Flow Cytometry （ FCM） after 24 h,also,we took the photofluorography of the apoptotic cells.Then the results were analyzed with one-way anova .Results and conclusion： The growth of cultured retinal Muller cells were significantly restrained by ApoAI and this effect was concentration dependent in a certain range （P〈0.05）;ApoAI had no significant effect on apoptosis （P〉0.05）.