GC法同时测定解热消炎胶囊中4种挥发性组分

Simultaneous determination of four volatile constituents in Jiere Xiaoyan Capsules by GC

目的 建立GC法同时定量测定解热消炎胶囊中薄荷脑、异龙脑、龙脑、百秋李醇.方法 采用HP-20M毛细管柱（25 m×0.32 mm,0.3 μm）;升温程序为100℃保持2 min,以5℃/min升温至130℃,再以10℃/min升温至220℃,保持5 min;进样口温度为250℃;FID检测器温度为250℃;载气为高纯氮气,体积流量为2.0 mL/min,空气体积流量为400 mL/min,氢气体积流量为40 mL/min.结果 薄荷脑、异龙脑、龙脑、百秋李醇分别在0.081～0.406 mg/mL、0.052～0.262 mg/mL、0.054 ～0.270 mg/mL、0.013 ～0.067 mg/mL范围内具有良好线性关系.平均回收率分别为98.6％、99.5％、100.1％、96.9％,RSD分别为1.6％、0.7％、1.7％、1.3％.结论 本法进一步完善解热消炎胶囊质量控制.

AIM To establish a gas chromatography （GC） method to simultaneously determine the contents of menthol,isoborneol,borneol and patchouli alcohol in Jiere Xiaoyan Capsules.METHODS The separation was achieved on HP-20M column （25 m ×0.32 mm,0.3 μm） with its temperature maintained at 100 ℃ after injection for 2 min,and programmed to 130 ℃ at the rate of 5 ℃/min.Once the temperature was elevated to 220 ℃ at the rate of 10 ℃/min,held to it for 5 min.The temperature of flame ionization detector and the injection was set at 250 ℃.Highly pure nitrogen was used as the carrier gas.The flow rates of the carrier gas,air and hydrogen were 2.0 mL/min,400 mL/min and 40 mL/min,respectively.RESULTS Good linearities of menthol,isoborneol,borneol and patchouli alcohol were obtained within the ranges of 0.081-0.406 mg/mL,0.052-0.262 mg/mL,0.054-0.270 mg/mL and 0.013-0.067 mg/mL,respectively.The average recoveries were 98.6％,99.5％,100.1％ and 96.9％,and the RSDs were 1.6％,0.7％,1.7％ and 1.3％,respectively.CONCLUSION The method is a supplement to the quality control of Jiere Xiaoyan Capsules.