EB病毒诱发淋巴瘤中EB病毒核抗原-1的表达

Expression of EBNA-1 in EB virus-induced lymphomas

目的:检测EB病毒(EBV)诱导正常人淋巴细胞发生肿瘤前后EB病毒核抗原-1(EBNA-1)的差异表达情况,为研究EBV诱发淋巴瘤的发生机制提供实验依据。方法:构建SCID小鼠体内EBV诱发淋巴瘤的动物模型,分别采用实时定量PCR和Western blot方法检测EBNA-1基因的表达情况。结果:成功复制EBV诱发淋巴瘤动物模型,EBNA-1基因在6例"正常人"淋巴细胞和3例诱发淋巴瘤细胞中均有表达,在诱发淋巴瘤细胞中的表达比在正常人淋巴细胞中的表达上调11 683倍,差异有统计学意义。Western blot结果显示,与正常人淋巴细胞组相比,EBNA-1在诱发淋巴瘤细胞的蛋白表达明显升高。结论:EBV诱发淋巴瘤中EBNA-1的表达上调,EBNA-1在EB病毒诱发淋巴瘤的形成过程中可能起重要作用。

Objective To compare the different expression of EBNA-1 in the normal human lymphocytes and in the EBV-induced lymphomas so as to provide the basis for studying the mechanism of the EB virus- induced lymphoma. Methods Animal model of EBV-induced lymphoma was constructed in SCID mice. EBNA-1 gene expression level was detected by real-time PCR and Western blot. Results EBV-induced lymphomas in animal model were replicated. EBNA-1 gene was expressed both in normal human lymphoma cells and 3 EBV- induced lymphoma cells. In EBV-induced lymphoma cells, the expression was up-regulated 11 683 times than in normal lymphocytes. Western blot results showed that the expression of EBNA-1 protein in induced lymphoma cells increased significantly compared with that in normal lymphocytes. Conclusions EBNA-1 expression in EBV-induced lymphoma cells is up-regulated than in normal lymphocytes. EBNA-1 may play an important role in the development of EBV-induced lymphoma.