ALOX5AP基因SG13S114A/T和COX-2基因765G/C交互作用增加脑梗死的易感性

The interaction between ALOX5AP SG13S114A / T and COX-2 765G / C increases susceptibility to cerebral infarction

目的:探讨ALOX5AP SG13S114A/T、COX-2 765G/C和COX-1 50C/T多态性及交互作用与脑梗死的相关性。方法:采用聚合酶链反应-单核苷酸多态性方法对411例脑梗死患者(脑梗死组)和411名对照者(对照组)ALOX5AP基因SG13S114A/T、COX-2基因765G/C和COX-1基因50C/T位点多态性进行检测,应用广义多因子降维法(GMDR)检测基因与基因的交互作用。结果:ALOX5AP SG13S114 A/T、COX-1 50C/T和COX-2 765G/C基因型及等位基因分布在脑梗死组与对照组比较差异均无统计学意义;但GMDR示SG13S114和COX-2具有联合交互作用,同时携带ALOX5AP SG13S114AA型和COX-2 765CC基因型者脑梗死风险增加2.842倍。结论:对基因与基因的交互作用进行分析有助于更深入研究复杂疾病的基因型和表型间的关系。

Objective To investigate the interrelations of ALOX5AP SG13S114A/T , COX-2 765G/C , COX-1-50C/T polymorphisms and cerebral infarction. Methods The ALOX5AP SG13S114A/T, COX-2 765G/C and COX-1 50C/T polymorphisms in 411 cases with cerebral infarction and 411 controls were measured by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism method. The generalized multifactor dimensionality reduction （GMDR） method was employed to detect gene-gene interactions. Results Single-gene analysis showed that there were no significant differences in the genotype and allele frequency distributions of ALOX5AP SG13S114A/T, COX-2 765G/C and COX-1 50C/T between two groups. However, in those cases carrying ALOX5AP SG13S114AA as well as COX-2 765CC , the risk of cerebral infarction increased significantly by 2.842 times. Conclusions The combinational analysis among genes used in this study may be helpful in the elucidation of genetic risk factors for common and complex diseases.