THBS2表达与胃癌血管生成以及预后的关系

Relations of THBS2 expression with angiogenesis and prognosis of gastric cancer

目的:探讨胃癌组织中血小板反应蛋白2(THBS2)的表达及其与胃癌血管生成、预后的关系。方法:采用组织芯片和免疫组化技术检测120例胃癌和36例癌旁正常胃黏膜组织中THBS2、VEGF、MMP-2、MMP-9的表达以及CD34标记微血管密度(MVD)。同法检测总生存期≥10年的40例胃癌患者癌组织(长生存期组)和总生存期≤3年的30例胃癌患者癌组织(短生存期组)的THBS2的表达,比较两组患者的THBS2表达差异。结果:THBS2在胃癌组织中阳性表达率明显低于正常胃黏膜组织(P<0.05),而VEGF、MMP-2、MMP-9的阳性表达率及MVD值明显高于正常胃黏膜组织(均P<0.05);THBS2阳性胃癌组织中MVD值明显低于THBS2阴性胃癌组织,而VEGF阳性胃癌组织中MVD值明显高于VEGF阴性胃癌组织(均P<0.05);胃癌组织中THBS2的表达与VEGF、MMP-2、MMP-9呈明显负相关(r=-0.574、-0.447、-0.599,均P<0.01)。长生存期组患者胃癌组织THBS2的阳性表达率明显高于短生存期组(P<0.05)。结论:THBS2在胃癌中低表达,从而可能通过促进VEGF、MMP-2、MMP-9的表达影响胃癌的血管生成和预后。

Objective： To investigate thrombospondin 2 （THBS2） expression in gastric cancer tissue and its relations with the angiogenesis and prognosis of gastric cancer. Methods： Using tissue microarray and immunohistochemical technolog~ the expressions of THBS2, VEGF, MMP-2 and MMP-9, and the CD34-1abeled microvessel density （MVD） in 120 specimens of gastric cancer tissue and 36 specimens of tumor adjacent normal gastric mucosal tissue were detected. In addition, the THBS2 expression in the tumor tissues from 40 gastric cancer patients with the overall survival time 〉 10 years （long survival group） and 30 gastric cancer patients with the overall survival time 〈3years （short survival group） wasdetermined by the same method, and the THBS2 expression difference between the two groups was compared. Results： In gastric cancer tissue, the positive THBS2 expression rate was significantly lower while the positive expression rate of VEGF, MMP-2 and MMP -9 and the MVD value were significantly higher than those in normal mucosal tissue （all P〈0.05）. The MVD value in gastric cancer tissues with positive THBS2 expression was significantly lower than those with negative THBS2 expression, which in gastric tissues with positive VEGF expression was significantly higher than in those with negative VEGF expression （both P〈0.05）. THBS2 expression had negative correlation with the expressions ofVEGF, MMP-2 and MMP-9 in gastric cancer tissues （r=-0.574, -0.447, and -0.599, all P〈0.01）. The positive THBS2 expression rate in gastric cancer tissues in long survival group was significantly higher than that in gastric cancer tissues in short survival group （P〈0.05）. Conclusion： THBS2 expression is down-regulated in gastric cancer tissue, which may affect the angiogenesis and prognosis of gastric cancer through promoting the expressions ofVEGF, MMP-9 and MMP-2