中频脉冲电磁场照射骨髓间充质干细胞的拉曼光谱

Raman spectroscopy of bone marrow mesenchymal stem cells in medium-frequency pulsed electromagnetic fields

背景:采用中低频脉冲电磁场干预骨髓间充质干细胞增殖分化的研究很多,但采用表面拉曼光谱方法分析受电磁场照射单个干细胞的拉曼光谱罕有报道。目的:比较3 000 Hz中频脉冲电磁场照射与无电磁照射后存活状态下的骨髓间充质干细胞拉曼光谱的差异。方法:分离培养鉴定SD大鼠骨髓间充质干细胞,取第3代细胞接种到六孔板,分为2组:无电磁组和3 000 Hz中频脉冲电磁照射组。培养7 d后,将2组细胞分别转移到生理盐水中,各取30个细胞,每个细胞收集细胞周围3个点和中间一个点的拉曼光谱,利用每组所得120个数据计算出拉曼光谱的平均相对强度,进行2组对比。结果与结论:电磁场照射组和无电磁组骨髓间充质干细胞拉曼光谱特征峰基本相同,且origin软件作图后波形基本相同;电磁场照射组的峰值普遍较无电磁场组下降。提示激光镊子拉曼光谱技术能够应用于单个干细胞分子层面生物化学变化的检测和研究。与未经电磁场照射组相比,经3 000 Hz中频脉冲电磁场照射后,骨髓间充质干细胞的拉曼光谱有明显差异,峰值普遍降低。

BACKGROUND：Studies about low-frequency pulsed electromagnetic fields interfering with bone marrow mesenchymal stem cells proliferation and differentiation are many, but the Raman spectra of single stem cells irradiated in electromagnetic fields analyzed by surface Raman spectroscopy analysis are rarely reported. OBJECTIVE：To compare the difference in Raman spectra of bone marrow mesenchymal stem cells with or with no irradiation of 3 000 Hz pulsed electromagnetic fields. METHODS：Bone marrow mesenchymal stem cells isolated from Sprague-Dawley rats were cultured and identified. Passage 3 cells were inoculated into 6-wel plates and divided into two groups：pulsed electromagnetic field irradiation group and blank control group. After cultured for 7 days, cells in the two groups were transferred to physiological saline, and 30 cells were randomly col ected from each group. Four Raman spectra were harvested from each celland the average relative intensity of Raman spectra was calculated and compared between two groups. RESULTS AND CONCLUSION：There were the same Raman peaks in the two groups, and the waveforms were basical y same in the two group based on the curve mapping by origin 7.0 software. The peak value in the irradiation group was decreased compared with the blank control group. Laser optical tweezers Raman spectroscopy can be applied to study the biochemical changes of a single stem cellat the molecular level. The Raman spectra of bone marrow mesenchymal stem cells irradiated by 3 000 Hz pulsed electromagnetic fields differ from those without irradiation, and the peak also lowered after irradiation.