补肾健脾化瘀方对去势大鼠股骨骨髓ERRα和PGC-1α mRNA表达的影响

Effect of kidney-tonifying,spleen-strengthening,and blood stasis removing recipe on the expression ERRα and PGC-1α mRNA in the bone marrow of the femurs in ovariectomized rat model

目的探讨补肾健脾化瘀方对去势大鼠股骨骨髓组织中雌激素相关受体α(ERRα)和过氧化物酶体增殖物活化受体γ协同刺激因子-1α(PGC-1α)mRNA表达的影响。方法 40只7月龄SD雌性大鼠随机分成假手术组、模型组、中药组(补肾健脾化瘀方)、阿伦膦酸钠组。治疗12w后,小动物双能X线检测左侧股骨骨密度,HE染色观察胫骨近端骨形态,QPCR检测骨髓组织中ERRα、PGC-1αmRNA表达水平检测。结果模型组骨密度低于假手术组(P<0.05);中药组与阿伦膦酸钠组骨密度高于模型组(P<0.05),而假手术组、中药组与阿伦膦酸钠组之间无统计学差异。与假手术组相比,模型组骨髓组织中ERRαmRNA表达水平上升(P<0.05),PGC-1αmRNA表达水平下降(P<0.05)。予以药物干预后,与模型组相比,中药组骨髓组织中ERRαmRNA表达水平下降(P<0.05),PGC-1αmRNA表达水平上升(P<0.05);而阿伦膦酸钠组骨髓组织中ERRαmRNA表达水平下降(P<0.01),PGC-1αmRNA表达水平虽呈现增高的趋势,但无统计学差异(P>0.05)。结论补肾健脾化瘀方可能是通过提高骨髓组织中PGC-1αmRNA表达水平,降低ERRαmRNA表达水平,从而提高去势大鼠骨密度。

Objective To explore the effect of kidney-tonifying,spleen-strengthening,and blood stasis removing recipe on the expression of ERRα and PGC-1α mRNA in the bone marrow of the rat femurs in an ovariectomized rat model.Methods Forty 7-month-old female SD rats were randomly divided into sham-operated group,ovariectomized（OVX） group,herb group（kidneytonifying,spleen-strengthening,and blood stasis removing recipe）,and alendronate（ALEN） group,with 10 rats per group.After a 12-week treatment,the bone mineral density（BMD） of the left femur was measured using small animal special dual energy X-ray absorptiometry.Bone morphology of the proximal tibia was observed using HE staining.The expression of ERRα and PGC-1αmRNA of bone marrow was detected using quantitative PCR.Results Compared with that in sham-operated group,BMD in OVX group was obviously decreased（P 0.05）.Compared with that in OVX group,BMD in herb group and in ALEN group was obviously increased（P 0.05）.There was no statistically significant among sham-operated,herb and ALEN groups.Compared with sham-operated group,the expression of ERRα mRNA in OVX group was obviously increased（P 0.05） and the expression of PGC-1α mRNA was obviously decreased（P 0.05）.After the drug intervention,the expression of ERRα mRNA in herb group was obviously decreased（P 0.05） and the expression of PGC-1α mRNA was obviously increased compared with that in OVX group（P 0.05）.The expression of ERRα mRNA in ALEN group was obviously decreased（P 0.01）,but PGC-1α mRNA expression increased with no statistical difference（P 0.05）.Conclusion The kidney-tonifying,spleen-strengthening,and blood stasis removing recipe enhances rat BMD by increasing the expression of PGC-1α mRNA and decreasing the expression of ERRαmRNA.