摘要
目的观察加味左归丸对体外培养的骨髓间充质干细胞(Mesenchymal stem cells,MSCs)向成骨细胞分化的影响。方法采用全骨髓法(贴壁筛选法)培养大鼠MSCs,用加味左归丸含药血清诱导大鼠MSCs向成骨细胞分化,并进行形态学观察和免疫细胞化学技术检测。结果与空白对照组相比,加味左归丸组与经典诱导组均可以明显促进成骨表型化MSCs的矿化结节形成及I型胶原表达(P<0.05)。结论加味左归丸可明显促进大鼠MSCs向成骨细胞转化,优化骨组织工程的种子细胞体系,改善骨组织的质量,促进骨组织的再生。
Objective To observe the effect of Zuogui pill on the osteoblast differentiation from mesenchymal stem cells（MSCs）in vitro.Methods MSCs from SD rats were cultivated by using whole marrow method.MSCs were induced to differentiate into osteoblasts by addition of conditioned serum of Zuogui pill.Morphology was observed and Immunocytochemistry was performed.Results Compared with the blank control group,mineralized nodule formation and type I collagen expression were promoted in both Zuogui pill group and classical induction group（P 0.05）.Conclusion Zuogui pill induces the differentiation of osteoblasts from MSCs.It can optimize the seed cell system,improve the quality of the bone tissue,and promote the regeneration of the bone tissue.
出处
《中国骨质疏松杂志》
CAS
CSCD
北大核心
2014年第10期1154-1158,共5页
Chinese Journal of Osteoporosis
基金
敦煌医学与转化省部共建教育部重点实验室开放基金项目(DHYX1213-004)
关键词
加味左归丸
中医中药
含药血清
骨髓间充质干细胞
成骨细胞
Zuogui Pill
Traditional Chinese medieine
Conditioned serum
Mesenchymal stem cells
Osteoblast
