β-TCP修复骨质疏松性骨缺损的实验研究

Experimental study on the repairing of osteoporotic bone defect using β-TCP

目的探索β-TCP修复骨质疏松性大鼠骨缺损的效果。方法取SD大鼠20只,先去卵巢建立骨质疏松模型,等饲养3个月保证骨质疏松模型成功后再建立双侧大鼠股骨干骺端缺损模型,将手术成功的骨质疏松性骨缺损模型随机均分为两组,β-TCP植入组于骨缺损处植入β-TCP,空白对照组不作任何处理。术后4,8w进行大体观察、X射线摄片、组织病理学观察,Micro-CT观察。结果术后4、8w,β-TCP植入组:缺损区X射线影像有明显的骨组织生成,HE染色可见大量骨小梁和较多核深染的长梭形骨细胞,Micro-CT显示骨微观结构修复效果较好,且能明显增加股骨骨密度;空白对照组X射线骨组织生成较少,骨小梁或骨板结构较少,排列较紊乱,Micro-CT显示骨微观结构修复效果不够理想,股骨骨密度增加不明显。结论β-TCP可较好地修复骨质疏松性大鼠股骨干骺端缺损。

Objective To investigate the role of β-TCP in bone defect repairing in osteoporotic rats.Methods Twenty SD rats were ovariectomized and fed for 3 months to establish the osteoporosis model.Subsequently,bone defects were created in bilateral femoral metaphysis by surgery.Successful osteoporosis bone defect models were divided into 2 groups,including β-TCP group and control group.β-TCP was embedded into bone defect in β-TCP group.No treatment was performed in control group.Gross observation,X-ray radiographs,tissue pathological observation,and Micro-CT observation were conducted 4 and 8 weeks after the surgery.Results X-ray radiographs showed that bone tissue growth was obvious in bone defect zone 4 weeks and 8 weeks after surgery in β-TCP group.HE staining showed that there were considerable amount of trabeculae and spindle shaped,deep stained osteoblasts.Micro-CT showed that bone micro-structure was comparatively well repaired,and the bone mineral density of the femur increased significantly.X-ray radiographs showed few bone growth,few trabeculae or bone lamella structure,and irregular distribution in control group.Micro-CT indicated that bone micro-structure was not desirably repaired,and the increase of bone mineral density of the femoral was not obvious.Conclusion β-TCP can comparatively well repair femoral metaphysis bone defect in osteoporotic rats.