不同方法制备移植修复材料对异体肌腱生物学性能的影响

Impact of different preparation methods for graft materials on biological properties of allogeneic tendon

背景:移植的肌腱必须具有良好的生物力学性能,才能有效避免缝合时肌腱吻合端的撕裂,减轻肌腱愈合过程中粘连程度。目的:探讨移植修复材料不同制备方法对异体肌腱生物学性能的影响。方法:将48只健康雄性来亨鸡依据随机数字表法分为均分为3组,切取3组鸡一侧第三趾屈趾浅深肌腱,玻璃化组、化学萃取组分别采用玻璃化法与化学萃取法处理肌腱,空白对照组肌腱不做任何处理。取3组一部分肌腱进行生物力学检测;另一部分进行异体移植,移植1,2,3,6周检测外周血CD4+、CD8+T淋巴细胞数。结果与结论:玻璃化法可保留部分原有肌腱细胞,化学萃取法不能。3组肌腱拉伸断裂强度、拉伸断裂功耗及拉伸断裂延伸率比较差异无显著性意义(P>0.05)。移植后1,2周末,3组间CD4+、CD8+、CD4+/CD8+比较差异有显著性意义(P<0.05);移植后3,6周末,玻璃化组、化学萃取组CD4+、CD8+、CD4+/CD8+均明显少于空白对照组(P<0.05),玻璃化组和化学萃取组间CD4+、CD8+、CD4+/CD8+比较差异无显著性意义(P>0.05)。表明采用玻璃化法和化学萃取法在有效保留肌腱生物力学性能的基础上,可显著降低肌腱的免疫原性。

BACKGROUND：The transplanted tendon must have good biomechanical properties, in order to effectively avoid tendon tear at the anastomosis end during suturing and reduce adhesion of tendon during healing process. OBJECTIVE：To investigate the effects of different methods for preparation of graft materials on the biological properties of tendon al ograft. METHODS：Forty-eight healthy male Leghorns were randomly divided into three groups：vitrification group, chemical extraction group, and control group. Unilateral superficial and deep flexor tendon of the third toe was subjected to vitrification, chemical extraction and no treatment in the three groups, respectively. A part of tendon was taken for biomechanical testing, and the other part was for al ogeneic transplantation. After 1, 2, 3, 6 weeks, peripheral blood CD4＋, CD8＋T lymphocytes were counted. RESULTS AND CONCLUSION：Vitrification could partial y retain the original tendon cells, but the chemical extraction method could not. Tensile strength for tendon rupture, tensile fracture power and tensile elongation at break were not statistical y significant among three groups （P〉0.05）. At the end of 1 and 2 weeks after transplantation, CD4＋, CD8＋, CD4＋/CD8＋difference was significant among the three groups （P〈0.05）;at the end of 3 and 6 weeks after transplantation, CD4＋, CD8＋, CD4＋/CD8＋were significantly less in the vitrification and chemical extraction groups than the control group （P〈0.05）, but no difference was found between the vitrification group and chemical extraction group （P〉0.05）. These findings indicate that the vitrification and chemical extraction methods can significantly reduce immunogenicity of the tendon based on effective retention of biomechanical properties of the tendon.