重症急性呼吸道感染儿童中人博卡病毒的分型检测与基因进化分析

Genotyping detection and phylogenetic analysis of human Bocavirus among inpatient children with severe acute respiratory infection

目的 了解重症急性呼吸道感染住院儿童中人博卡病毒（HBoV）的感染状况,流行病学特征及其进化特征.方法 采用巢式PCR的方法,对来自北京儿童医院重症急性呼吸道感染住院儿童的259份鼻咽抽吸物,进行人博卡病毒（HBoV）分型检测与测序,同时进行了合并感染检测、流行病学、临床特点及基因多态性分析.结果 共检出56份人博卡病毒感染阳性标本,阳性率为21.6％,[95％ CI（16.0％～27.3％）,P＜0.0001],其中2岁以下儿童感染率较高.与其他呼吸道常见病毒的合并感染率为94.6％.HBoV阳性产物测序分析发现,人博卡病毒1型占96.4％ （54/56）,2、3型各1份.HBoV阳性株分型区（VP1/VP2）序列变异不明显.结论 人博卡病毒是儿童急性呼吸道感染常见的病原体,以I型最为常见,分型区（VP1/VP2）序列较保守.HBoV在重症急性呼吸道感染儿童中是否起到真正的致病作用还需进一步的研究.

Objective To investigate the prevalence,epidemiological and phylogenetic characteristics of human Bocavirus （HBoV） among inpatient children with severe acute respiratory infection （SARI）.Methods Two hundred and fifty-nine nasopharyngeal aspirates （NPAs） were collected from inpatient children with SARI in Beijing Children＇ s Hospital.Human Bocavirus was genotypic detected by Nested PCR and sequenced followed by the epidemiological and phylogenetic analysis.Results HBoV was detected in 56 of 259 NPAs from inpatient children with SARI.The positive rate was 21.6%,95% CI （16.0%-27.3%）.Most of the HBoV infection children were younger than two years.The co-infection rate of HBoV with other respiratory viral pathogens was 94.6%.And 96.4% （54/56） were positive for HBoV1.Only one of HBoV2 and one of HBoV3 positive case was found.The sequence evolution of partial VP1/VP2 fragment was relative conserved by phylogenetic analysis,Conclusion HBoV was common detected viral pathogens among children with SARI.HBoV1 was more prevalent than other genotype.However,more investigation was needed to determine the etiological role of HBoV infection among inpatient children with SARI.