轻度胃肠炎伴婴幼儿良性惊厥患儿T细胞活化亚群、细胞因子的变化

Changes in T cell activation and cytokines of benign infantile convulsions with mild gastroenteritis

目的 通过对轻度胃肠炎伴婴幼儿良性惊厥（benign infantile convulsions with mild gastroenteritis,BICE）患儿T细胞活化亚群、细胞因子的检测,探讨其在BICE发病机制中的可能作用.方法 选择2012年4月至2013年4月浙江大学医学院附属儿童医院神经科病房收住院的31例BICE患儿为病例组,同期门诊体检的23例健康儿童为对照组,通过流式细胞术检测两组外周血CD25 ＋/CD3＋ CD4＋、CD69 ＋/CD3＋ CD4＋、HLA-DR/CD3＋ CD4＋、CD25 ＋/CD3＋ CD8＋、CD69＋/CD3＋ CD8＋、HLA-DR/CD3＋ CD8＋,及IL-2、IL-4、IL-6、IL-10、TNF-α、INF-γ水平.结果 病例组外周血HLA-DR/CD3＋CD4＋、HLA-DR/CD3＋CD8＋的表达含量（7.300％±2.725％,29.560％±14.079％）高于对照组（t=2.110,2.649,P=0.040,0.011）,外周血IL-10的表达水平为8.100（5.800～ 19.700） pg/ml,与对照组相比,差异有统计学意义（z=-3.342,P＜0.01）.结论 BICE患儿存在一定程度的免疫功能紊乱,T细胞活化亚群和细胞因子可能参与了BICE的发病过程.

Objective To explore the action of immune function in benign infantile convulsions with mild gastroenteritis （BICE） pathogenesis by detecting the T cell activation and cytokine of BICE.Method Thirty-one infant patients with BICE hospitalized in the wards of department of neurology of The Children＇ s Hospital Zhejiang University School of Medicine from April 2012 to April 2013 were selected as the experiment group,while 23 healthy infants accepting health check-up in the same period were selected as the control group.Flow cytometry was adopted to detect the peripheral blood CD25 ＋/CD3 ＋ CD4 ＋,CD69 ＋ /CD3 ＋CD4 ＋,HLA-DR/CD3 ＋ CD4 ＋,CD25 ＋/CD3 ＋ CD8 ＋,CD69 ＋/CD3 ＋ CD8 ＋,HLA-DR/CD3 ＋ CD8 ＋,and IL-2,IL-4,IL-6,IL-10,TNF-α,INF-γlevels of the two groups.Results The peripheral blood HLA-DR/CD3 ＋ CD4 ＋,HLA-DR/CD3 ＋ CD8 ＋ expression levels （7.300% ＋ 2.725%,29.560%＋ 14.079%） of the experiment group were higher than those of the control group （t =2.110,2.649,P =0.040,0.011）; the expression level of the peripheral blood IL-10 was 8.100 （5.800-19.700） pg/ml,which was significantly higher than that of the control group （z =-3.342,P 〈 0.01）.Conclusion There is a certain degree of immune dysfunction in BICE patients,and T cell activation subgroup and the cytokines may participate in the morbidity progress of BICE.