Toll样受体4对牙周膜细胞增殖的影响

Effect of toll-like receptor 4 on the proliferationin of human periodontal ligament cells

目的:了解Toll样受体4对人牙周膜细胞增殖的影响。方法:以改良组织块法体外获得人牙周膜细胞,取生长良好的第3代细胞随机分为3组:空白对照组、NC对照组(NC-shRNA转染组)和TLR4-shRNA转染组,并于转染48 h后用倒置荧光显微镜观察转染效果、用荧光实时定量PCR检测各组细胞中TLR4mRNA的表达水平;最后将3组细胞分别接种于96孔细胞培养板进行培养,分别于培养24、48、72 h用MTT法检测各组细胞的增殖能力。结果:TLR4-shRNA可明显降低人牙周膜细胞的TLR4mRNA表达水平(P<0.05);MTT检测结果显示,TLR4-shRNA转染组在常规培养48、72 h的OD值均明显低于空白对照组和NC对照组(P<0.05),而NC对照组的OD值在72 h时明显低于空白对照组(P<0.05)。结论:TLR4对牙周膜细胞的增殖有一定调控作用,转染慢病毒后该作用增强。

AIM：To examine the effects of toll like receptor 4 （TLR4）on the proliferation of human perio-dontal ligament cells （hPDLCs）.METHODS：Primary hPDLCs were cultured by tissue block enzymolytic method. Cells of Passege 3 were transfected with NC-shRNA and TLR4-shRNA respectively,cells without treatment served as the controls.72 hours after treatment,TLR4 mRNA expression was examined by real-time quantitative polymerase chain reaction （RT-PCR）.MTT assay was used to test the proliferation of hPDLCs.RESULTS：TLR4 mRNA expres-sion in the TLR4-shRNA group was significantly decreased than that in other groups （P〈0.05）.MTT assay showed that the OD values of TLR4-shRNA group at 48 and 72 h were significantly lower than those of the NC-shRNA group or blank control group（P〈0.05）.CONCLUSION：TLR4 interference suppresses cell proliferation of hPDLCs.