烟酰胺对角质形成细胞吞噬黑素体抑制的实验观察

Inhibition of Niacinamide on Keratinocytes Phagocytizing Melanosomes

目的探索角质形成细胞(keratinocyte,KC)吞噬黑素体(melanosome,MS),并验证烟酰胺对吞噬的抑制作用。方法从人毛发中提取的MS经透射电镜检测后,加到角质形成细胞中,观察MS是否被吞噬,用HMB-45抗体免疫细胞化学染色检测。同时利用Millicell建立了黑素细胞(melanocyte,MC)和角质形成细胞共培养模型,用HMB-45荧光抗体标记KC吞噬的MS,并分别加烟酰胺进行干预。结果角质形成细胞能吞噬从人毛发中提取的MS,在共培养模型中,MC产生的MS可以通过孔径为8μm的孔膜,并被其下的KC所吞噬。两者均验证了烟酰胺抑制吞噬作用。结论从毛发中提取的MS及Millicell共培养模型可以用于MS转运的研究中,并为MS转运的研究提供新思路。

Objective To explore the phagocytizing process of melanosome by keratinocytes, and to verify the inhibitory effect of niacinamide, Methods After melanosomes were isolated from human hair and further confirmed by a transmission electron microscope, they were added to the keratinocyte cultures. Millicell cell culture plates were used to establish the keratinoeyte-melanocyte co-culture model. To determine whether niacinamide inhibited keratinocyte phagocytosis, niacinamide was added to the cell cultures. Immunostaining with HMB45 antibody was used to determine whether melansomes were phagocytized by keratinocytes. Results Keratinocytes could phagocytize the melanosomes from human hair. In the keratinocyte-melanocyte co-cuhure model, the melanosomes secreted by melanocytes could pass through the 8μm-pore of Millicell plate, and were phagocytized by keratinocytes under the Millicell. Niacinamide could inhibit the phagocytosis in both co-culture and keratinocyte culture. Conclusion Melanosomes from hair and Millicell model could be used to study the transfer of melanosomes, providing a new idea for future studies.