广州地区2013年淋球菌耐药监测结果分析

Analysis of Antibiotic Resistance of Neisseria Gonorrhoeae Isolated from Guangzhou in 2013

目的了解广州地区淋球菌对7种抗生素耐药性及PPNG和TRNG的流行状况。方法用琼脂稀释法测定头孢曲松、头孢克肟、青霉素、大观霉素、环丙沙星、阿奇霉素和四环素的最低抑菌浓度(MIC);用纸片碘量法检测β-内酰胺酶。结果 100株淋球菌检出PPNG 33株(33%)、TRNG 40株(40%),青霉素、环丙沙星、阿奇霉素的耐药率分别为93%,98%,17%,检出环丙沙星高度耐药株42株(42%),未发现头孢曲松、头孢克肟耐药菌株,但其低敏率分别为12%,11%,未发现大观霉素耐药菌株和低敏菌株。结论广州地区分离淋球菌对青霉素、环丙沙星耐药严峻,但对头孢曲松、头孢克肟、大观霉素均敏感,需对药物耐药性进行动态监测以指导临床合理应用抗菌素。

Objective To survey the antibiotic resistance to 7 antibiotics and the prevalence of penicillinase-producing Neisseria gono,Thoeae （PPNG） and Tetracycline-Resistant Neisseria gonorrhoeae （TRNG） in Guangzhou. Methods Minimum inhibitory concentrations （MIC） of ceftriaxone,cefixime, penicillin, spectinomycin, ciprofloxacin, azithromycin, and tetracycline were determined by agar dilution method. β-lactamase was measured by paper iodine quantitative method. Results Thirty-three out of 100 strains were PPNG（33% ） and 40 out of 100 strains were TRNG （40%）. The rates of resistance to penicillin, ciprofloxacin, and azithromycin were 93% , 98 % , and 17%, respectively. Forty-two highly ciprofloxacin-resistant strains were detected. Although no ceftriaxone or cefixime resistant strains were detected, the rates of low susceptibility to ceftriaxone and cefixime were 12% and 11%, respectively. No spectinomycin-resistant strains and its low susceptibility were detected. Conclusion Neisseria gonorrhoeae is highly resistant to penicillin and ciprofloxacin in Guangzhou,but sensitive to ceftriaxone, cefixime and spectinomycin. It is necessary to survey the antibiotic resistance of Neisseriae gonorrhoeae in order to properly use antibiotics.