rHSG对COPD大鼠小气道阻力及成纤维细胞TGF-β1表达的影响

Effects of rHSG on small airway resistance and the expression of TGF-β1 in fibroblasts of COPD rats

目的检测正常COPD大鼠小气道成纤维细胞中r HSG的表达,寻找其与小气道阻力、TGF-β1的相关性,探索r HSG调控气道重塑的机制。方法将大鼠随机分为对照组及模型组,分别检测肺功能和肺泡灌洗液中TGF-β1含量。用组织块贴壁法培养气道成纤维细胞,用RT-PCR和Western blot检测r HSG mRNA和蛋白的表达。分析r HSG基因量与小气道阻力以及TGF-β1的相关性。结果模型组大鼠先后出现咳嗽、气急、喘鸣和痰多等症状并伴有精神萎靡、纳少和毛发枯黄等,之后出现体质量减轻,肺体积略增大,肺顺应性明显下降,而气道阻力明显高于对照组(P<0.01);肺泡灌洗液中TGF-β1含量均明显高于对照组(P<0.01);r HSG基因在对照组中表达高于模型组(P<0.05);r HSG mRNA和蛋白与气道阻力(RI)、TGF-β1呈负相关,与肺顺应性(cydn)呈正相关。结论 r HSG可能通过调控TGF-β1水平干预气道重塑过程。

Objective By testing the normal rat,rat COPD model,the expression of small airway fibroblasts rHSG was detected to look for the correlation of small airway resistance,TGF-β1 and rHSG,to explore rHSG regulation mechanism of airway remodeling.Methods Rats were randomly divided into normal group and model group to test lung function and TGF-beta 1 contenting in alveolar lavage fluid respectively.Tissue culture RT-PCR and Western blot were performed.Results Module rats have a cough,wheeze,stRidor and phlegm syndrome accompanied by depression,diet reduce,hair yellow,and after weight loss,lung volume increases slightly,lung compliance decreased obviously,and airway resistance was significantly higher than the normal group （P ＜ 0.01） ; TGF-β1 contented in alveolar lavage fluid was significantly higher than that of normal group （P ＜0.01） ; rHSG genes expressed in normal group was higher than building group （P ＜ 0.05）,rHSG mRNA and protein were negatively correlated with RI,TGF-β1,and were positively correlated with Cdyn.Conclusions rHSG may regulate the TGF-β1 level to intervent airway remodeling process.