达玛烷苷元对睡眠干扰所致小鼠学习记忆障碍的改善作用

Improving effects of dammarane sapogenins on sleep interruption-induced learning and memory impairment in mice

目的研究达玛烷苷元(dammarane sapogenins,DS-1226)对睡眠干扰(sleep interruption,SI)致认知障碍小鼠学习记忆能力的影响。方法将ICR小鼠随机分为对照组,模型组,DS-1226低、中、高三个剂量组。用改良滚筒法对小鼠进行睡眠干扰,同时灌胃给药15 d后,用自主活动仪检测自主活动,水迷宫、避暗实验检测学习记忆能力。结果与模型组比较,DS-1226各给药组总路程、平均速度、运动总时间均增加。模型组水迷宫定位航行D5、D6潜伏期增加;与模型组和低剂量组比较,DS-1226中剂量组D6潜伏期降低,高剂量组D1-D6潜伏期降低;与中剂量组比较,DS-1226高剂量组D1-D5潜伏期降低。模型组水迷宫空间探索目标象限穿台次数及游程比率均低于对照组;DS-1226高剂量组目标象限穿台次数、目标象限游程比率及时间比率均高于模型组,目标象限穿台次数高于中剂量组,目标象限游程比率高于低剂量组。模型组避暗学习错误次数、暗室路程、暗室时间、暗室静息时间增加;与模型组比较,DS-1226低、中剂量组错误次数、暗室路程减少,高剂量组错误次数、暗室时间、暗室路程、暗室静息时间减少,明室时间增加。结论水迷宫和避暗实验结果表明DS-1226给药对SI所致小鼠学习记忆障碍有改善作用,且有剂量相关性。

Objective To study the effects of dammarane sapogenins （ DS-1226 ） on sleep interruption-induced learning and memory impairment in mice.Methods 130 SPF healthy 5 -6-week old male ICR mice were randomly divided into control, model, DS-1226 low dose, DS-1226 medium dose and DS-1226 high dose groups.The behavioral alterations in open field （OF）, Morris water maze （MWM） and step-through （ST） tests were detected at 15 days after rotating drum-induced sleep interruption （ SI） .Results The total distance, movement speed, total duration of movement were increased in OF test （ P〈0.05, vs.the model group） after treatment.The latency of place navigation was increased from day 5 in the MWM test after 15 d sleep interruption, and the number of crossing in the target quadrant and the percent distance in target quadrant were decreased after 15 d sleep interruption （ P 〈0.05, vs.the control group）, while the latency of place navigation was decreased, and the percent distance in target quadrant and percent time in target quadrant after high dose DS-1226 oral administration （ P〈0.05, vs.the model group） were increased.Error times, distance in dark chamber, time in dark chamber and immobility time in dark chamber were increased in training of step through test （ P〈0.05, vs.the control group）;while these indexes were decreased after DS-1226 oral administration （ P〈0.05, vs.the model group） .But there was no significant difference in the step through testing course.Conclusions The results show that orally administrated DS-1226 can ameliorate SI-induced learning and memory impairment, and there is a significant dosage-effect relationship.