二苯乙烯苷对癫痫大鼠神经细胞增殖的影响及其机制探讨

Effect and mechanisms of tetrahydroxystilbene glucoside on neuron proliferation in the hippocampus of epileptic rats

目的观察二苯乙烯苷(tetrahydroxystilbene glucoside,TSG)对癫痫(epilepsy,EP)大鼠神经细胞增殖的影响,并探讨其可能机制。方法将SD大鼠随机分为假手术组、模型组、二苯乙烯苷干预组。应用立体定向脑室内微量注射的方法建立海人酸(kainic acid,KA)诱导的大鼠癫痫模型。造模前30 min腹腔注射给药,致痫后6 h腹腔内注射TSG溶液(3 mg/kg)剂量,次日开始每日8时给予腹腔注射TSG溶液(3 mg/kg)剂量,每日1次,连续注射,共注射42 d,KA模型组动物及假手术组给予等量生理盐水腹腔内注射。用免疫组织化学技术观察TSG对大鼠海马齿状回颗粒细胞下层BrdU阳性细胞数目及海马齿状回区星形胶质细胞数目增殖的影响。结果癫痫发生后各组海马齿状回区及皮层均存在GFAP免疫反应阳性细胞,经统计分析二苯乙烯苷干预组较模型组GFAP免疫反应阳性星形胶质细胞减少,差异有统计学意义(P<0.01);BrdU免疫组织化学染色研究显示二苯乙烯苷干预组较假手术组及模型组海马齿状回区细胞增殖数目增加(P<0.01),差异有显著统计学意义。结论二苯乙烯苷存在抑制大鼠海马区星形胶质细胞增生,促进增神经细胞增殖的作用。

Objective To explore the influence of tetrahydroxystilbene glucoside （ TSG ） on the neuron proliferation in epileptic rats and the related mechanisms.Methods 54 healthy SD male rats were randomly divided into three groups： sham-operated group, epilepsy group and epilepsy with TSG treatment group.The epilepsy group was established by stereotactic brain trace injection with kainic acid （ KA ） . TSG solution （ 3 mg/kg ） was injected intraperitoneally at 6 hours after the epilepsy group established, and then q.d.for consecutive 42 days.The sham-operated group and epilepsy group were injected with normal saline.The influence of TSG on cell proliferation of rat hippocampal dentate gyrus BrdU-positive granular cells was observed by immunohistochemistry.Results Compare with the epilepsy group, the amount of glial fibrillary acid protein （ GFAP）-positive astrocytes was significantly reduced and dentate gyrus BrdU-positive cells were significantly increased in the TSG group （ P ＆lt;0.01 ） .Conclusions Tetrahydroxystilbene glucoside （ TSG） promotes neurogenesis of neural stem cells and neurons, and inhibits the growth of hippocampal dentate gyrus astrocytes in epilepsy rats.