昆明山海棠内生真菌ThF-11化学成分抗菌杀线虫作用的研究

Antibacterial and nematicidal activity of endophyte Th F-11 of Tripterygium hypoglaucum( Levl. ) Hutch

目的研究昆明山海棠[Tripterygium hypoglaucum(Levl.)Hutch]内生真菌化学成分及其抗菌杀线虫活性。方法分离、纯化内生真菌Th F-11,利用基因测序对分离的昆明山海棠内生真菌进行鉴定。应用层析法和波谱法对其代谢产物进行分离纯化、结构鉴定。分别采用生物活性测定法、抑菌圈法和试管法测定杀线虫和抑菌活性。结果经ITS鉴定昆明山海棠内生真菌Th F-11为简青霉(Penicillium simplicissimum),波谱法鉴定化合物结构为Penicillic acid[1]、2,4-dimethoxy-6-methylphenol[2]和5(6)-dihydropenicillic acid[3]。杀线虫活性显示化合物[1]随着时间、浓度增加活性逐渐增强,且对大肠杆菌、蜡样芽孢杆菌、枯草芽孢杆菌、金黄色葡萄球菌和绿脓杆菌均具有较强的抑菌活性。结论首次从内生真菌Th F-11获得3个化合物,且化合物[1]具有较强的抑菌、杀线虫的生物活性,值得进一步开发应用。

Aim To investigate the chemical compo- nents and antibacterial and nematicidal activity of en- doparasitic fungi ThF-11 of Tripterygium hypoglaucum （Levl.） Hutch. Methods The endophyte ThF-11 was isolated, purified and identified based on fungal ITS sequence. The chemical components were isolated and[1], 2,4_dimethoxy-6-methylphenol [2] and 5 （6）- dihydropenicillic acid [3] based on spectral data. Compound [1] showed significant activity against Cae- norhabditis elegans in a dose-dependent manner, and had antibacterial activity to Escherichia coli, Bacillus cereus, Bacillus subtilis, Staphylococcus aureus and Pseudomonas aeruginosa. Conclusion These corn-identified with column chromatography and NMR spec- trometer, respectively. The nematicidal and antibacte- rial activity was investigated by biological active meth- od and inhibition zone method. Results The endo- phyte ThF-11 was identified as Penicillium simplicissi- mum, the compounds were identified as Penicillic acidpounds are firstly isolated from endophyte ThF-11 of T. hypoglaucum （Levi.） Hutch. Compound [1] shows strong nematicidal activity and antibacterial activity, and is worth further studying and developing.