基于ISSR技术研究诺丽种质资源的亲缘关系

Genetic Relationship of Morinda citrifolia Germplasms by ISSR

为探讨我国诺丽（Morinda citrifolia）种质资源的亲缘关系,采用ISSR 技术对诺丽种质资源的遗传关系进行分析.结果表明, 10 条ISSR 引物对13 份诺丽种质资源共扩增出183 条带,其中多态性条带有159 条,占86.9%.13 份诺丽种质的遗传相似系数为0.464-0.784.聚类分析将13 份诺丽种质资源聚为两类,其中诺丽小黑种单独聚为一类,与其他12 份诺丽种质资源的亲缘关系较远.虽然按照外部形态特征不能将所有诺丽种质完全区分,但具有相同特征的多数种质还是聚在同-类或亚类中.

In order to understand the genetic relationship of Morinda citrifolia （noni）, the DNA fingerprintsof 13 noni germplasm resources of were studied by ISSR markers. The results showed that ten ISSR primerscould amplify 183 bands, of which 159 bands were polymorphic, accounting for 86.9%. The genetic similaritycoefficients of 13 noni germplasm resources ranged from 0.464 to 0.784. Cluster analysis showed that 13 nonigermplasm resources could be divided into two clads, in which Small fruit as a single group had distant relationwith other germplasm resources. Although all noni germplasm resources could not completely separated accordingexternal morphological characteristics, the most with the same characteristics could be clustered into the samegroup or sub-group.