山奈酚对四氯化碳致小鼠急性肝损伤的保护作用

Protective effects of kaempferol on CCl_4-induced acute liver injury in mice

目的研究山奈酚对四氯化碳(CCl4)致小鼠急性肝损伤的保护作用并探讨其作用机制。方法昆明种雄性小鼠随机分为空白对照组、模型组、阳性对照组(水飞蓟宾组)和低、高剂量山奈酚组。前2组给予溶剂蒸馏水灌胃,其余组分别给予0.1g/(kg·d)水飞蓟宾或6mg/(kg·d)、18mg/(kg·d)山奈酚,连续7d。末次灌胃2h后,空白对照组腹腔注射花生油,其余各组腹腔注射1.5mL/L CCl4花生油混合液,制备急性肝损伤模型。测定并比较各组小鼠血清谷丙转氨酶(ALT)和谷草转氨酶(AST),肝组织丙二醛(MDA)和超氧化物歧化酶(SOD)的含量或活力及肝脏病理学变化的差异。结果与模型组比较,山奈酚低、高剂量均可明显降低小鼠血清ALT和AST含量(P<0.05或P<0.01),降低肝组织MDA含量(P<0.01),增加SOD活力(P<0.05或P<0.01),水飞蓟宾对上述指标有相似作用(P<0.05或P<0.01)。病理切片显示,与模型组比较,水飞蓟宾组和低、高剂量山奈酚组的肝细胞坏死范围及程度均明显减少,炎细胞浸润程度明显减轻,其中高剂量山奈酚组的效果最好。结论山奈酚对CCl4致小鼠急性肝损伤有保护作用,其机制可能是山奈酚减少自由基的生成。

Objective To study the protective effects and mechanism of kaempferol on carbon tetrachloride （CC14）-induced liver injury in mice. Methods Kunming male mice were randomly divided into blank control group, model group, positive control group, and low- and large-dose kaempferol groups. The first two groups were given distilled water and the other groups were given silybin at 0.1 g/kg body weight/d, and kaempferol at 6 and 18 mg/kg body weight/d for 7 consecutive days, respectively. The blank control group was intraperitoneally injected with peanut oil, and the other groups were intraperitoneally given 0.15% CCL peanut oil solution at 2 h after the last administration in order to prepare acute liver injury mice models. The levels of serum alanine aminotransferase （ALT） and aspartate aminotransferase （AST）, and malondialdehyde （MDA） and superoxide dismutase （SOD） in liver tissue in all groups were determined and compared. Meanwhile, liver histopathology was investigated. Results Compared with model group, kaempferol could significantly decrease ALT and AST activities in the mice serum （P〈0.05 or P〈0.01） and MDA in the liver tissue （P〈0.01）, and increase the activity of SOD in liver tissue （P〈0.05 or P〈0.01）. Silybin had the similar actions. The pathologic examination showed that compared with those in model group, the range and degree of liver cell necrosis and inflammatory cell infiltration in silybin andkaempferol groups were significantly reduced, with high-dose kaempferol Oonclusion Kaempferol has a protective effect on CCl4-induced acute liver be related to reducing the generation of free radicals. group having the strongest effects injury in mice. The mechanisms may