去势大鼠劳损性膝骨关节炎新模型的建立

Establishment of Rat Knee Osteoarthritis Model via Ovariectomy Combined with Excessive Exercise

目的:通过模拟女性膝骨关节炎的主要病因,建立新动物模型,为进一步研究该病的机制奠定基础。方法:24只8月龄雌性SD大鼠随机分为空白对照组、假手术对照组和去卵巢组3组:去卵巢组大鼠行双侧卵巢切除术,假手术对照组切除卵巢周围脂肪组织,3组大鼠术后开展动物跑台运动训练。7周后处死,取膝关节及血清行组织学及免疫学分析。结果:去卵巢组大鼠膝关节呈现中度软骨损伤,与空白对照组比较,去卵巢组血清中iNOS浓度显著升高,(P<0.05)、SOD浓度显著下降(P<0.05);与假手术对照组比较,iNOS浓度升高程度更明显。结论:大鼠双侧卵巢切除后行疲劳运动创建的去势大鼠劳损性膝骨关节炎新模型稳定性高,符合临床更年期女性的骨关节炎病理,值得推广。

Objective：Knee Osteoarthritis（KOA） is associated with age-related loss of homeostatic balance.Our previous clinical studies have indicated that KOA is a kind of serious problem for most elder women.The aim of the present study is to establish a new rat knee osteoarthritis model via ovariectomy combined with excessive exercise in order to further determine its mechanism.Methods：Twenty-four 8-month female SD rats were randomly divided into blank group,sham-operated group（Sham Group）and ovariectomized group（OVX Group）.OVX group received bilateral ovariectomy,while only fat around the ovary was removed in the Sham group.Rats in all groups received downhill treadmill training for 5 weeks,200 minutes per day and 6 days per week.After 7 weeks,all rats were killed for observation of morphological change of the femoral condyle,tibial plateau and patella cartilage as well as immunological analysis.Results：OVX group showed mild osteoarthritic changes.Compared to blank group,the serum level of iNOS in OVX group was significantly increased（P〈0.05）,and SOD significantly decreased（P〈0.05）.The increase of serum iNOS was more obvious compared to SHAM group.Conclusion：The rat KOA model is successfully established by the method of bilateral ovariectomy combined with excessive exercise,which is stable and is in accordance with the osteoarthritic pathology of menopausal women.