超声波辅助溶菌酶酶解提取地木耳蛋白质工艺优化

Optimized extraction on crude proteins extraction from Nostoc commune by ultrasonication combined with hydrolyzation of muramidase

以优化地木耳蛋白质提取工艺为目的,研究地木耳的酶解、冻融及超声法联合破碎细胞的提取工艺,将蛋白质提取率作为考察指标,选取料液比、提取时间和提取功率3个主要因素对工艺条件进行优化。采用响应面分析(RSA)方法预测出地木耳蛋白质得率的回归模型;对回归模型进行分析,确定优化工艺条件为:时间27.5min、功率1 000W、料液比1∶42(m∶V),该条件下的蛋白质提取率为17.24%,显著高于常规提取方法。

To optimize the protein extraction process from Nostoc commune, a combined process of freezing and thawing, enzymatic hydrolyzation and ultrasonication was used to break Nostoc commune cells, and the major parameters of sample to ratio of material to liq- uid, extraction time and ultrasonic power were optimized with the yield of protein as the assessment indicator. A predicted regression model was established by using response surface analysis method and the optimum process conditions were obtained： sample to extraction time 27.5 rain, ultrasonic power 1 000 W, ratio of material to liquid 1 ： 42（m ： V）, of which the extraction yield was 17. 24%, significantly higher than that in traditional process.