长白山地区高山红景天中红景天苷含量及农药残留的测定

Determination of the content of salidroside and pesticide residues of Rhodiola Sachalinensis in Changbai Mountains

为了解长白山地区高山红景天药材的质量,对产自长白山地区不同年限的人工栽培和野生高山红景天中的红景天苷含量和农药残留量进行了测定.在检测波长为275nm、色谱柱为PLATISTLTM C18柱(250mm×4.6mm,5μm)、流动相为甲醇-乙酸铵溶液、线性度(r)高于0.999 8、平均回收率为96.62%、相对标准偏差为1.32%的条件下,采用高效液相色谱法测定了红景天苷的含量;在色谱柱为HP-5毛细管气相色谱柱(0.32mm×30m×0.25μm)、进样口温度为250℃、Ni63电子捕获检测器温度为300℃、尾吹15mL/min、柱温以10℃/min升至220℃并保持20min条件下,采用气相色谱法测定了农药残留.结果表明,不同年限人工栽培品的红景天苷含量有差异,且含量均低于野生品,样品中均无六六六、滴滴涕农药残留.

To comprehend the quality of Rhodiola sachalinensis the content of salidroside and pesticide residues of artificial cultivated and wild Rhodiola sachalinensis in Changbai Mountains were measured. High performance liquid chromatography （HPLC） method was established for determination of salidroside content. The detection wavelength was 275 nm. The column PLATISTLTM C18 column （250 mm× 4.6 mm, 5μm） and mobile phase （methanol： ammonium acetate） were used. The coefficient of correlation （r） exceed 0. 999 8, the average recovery and the relative standard deviation were 96. 620/00 and 1.32%, respectively. The amount of pesticide residues was determined by Gas Chromatography （GC）. The HP-5 capillary column （0.32 min× 30 m 0. 25μm） was chosen. The inlet temperature and Ni63 electron capture detector temperature were 250℃ and 300℃ respectively. The tail blowing was 15 mL/min and the column temperature was raised to 220 ℃ by 10 ℃/min, then maintained for 20 min. The results showed the salidroside content of artificial culti- vated Rhodiola sachalinensis in different growing years were distinction, which were lower than that of wild Rhodiola sachalinensis. The BHC and DDT pesticide residues not detected in the samples.