Folin-Ciocalteu法测定昭通葡萄及其葡萄酒总酚含量

Measure of Total Polyphenols Content in Zhaotong Grape and Grape Wine by Folin-Ciocalteu Method

[目的] Folin-Ciocalteu法测定葡萄总酚含量,为昭通葡萄生产及其酿制工艺提供科学依据.[方法]采用甲醇作溶剂,超声提取葡萄总酚,以Folin-Ciocalteu试剂和50 g/L Na2CO3溶液处理,在765 nm处测其吸光度.[结果]以没食子酸为标准,质量浓度在1～40mg/L范围内与吸光度线性关系良好（r =0.999 5）.平行测定（n=6）葡萄总酚含量,RSD在1.1％ ～1.6％,对葡萄和葡萄酒的加标回收率试验结果分别为98.8％、98.3％,RSD依次为1.8％、2.0％.[结论]该法操作简便,重复性好,结果准确可靠,可用于测定葡萄及葡萄酒中总酚含量.

[Objective] In order to provide scientific basis for production and brewing of Vitis vinifera in Zhaotong,the total polyphenol content in grapes was measured by Folin-Ciocalteu method.[Method] Using methyl alcohol as the solvent,total polyphenols of Vitis vinifera was extracted by supersonic method,then added Folin-Ciocalteu reagent and 50 g/L Na2CO3 solution,and measured the absorbancy at 765 nm.[Result] Basing on gallic acid,the mass concentration had an obvious linear relation （r =0.999 5） with absorbancy within the scope of 1-40 mg/L.The parallel determination （n =6） of total polyphenol results were that,when RSD was between 1.1％ to 1.6％,the adding standard recovery of Vitis vinifera and grapes wine were 98.8％,98.3％,RSD 1.8％,2.0％.[Conclusion] This method is convenient,repeatable and faithful,which can be used for determination of polyphenol in grapes and grape wine.