木薯同步糖化发酵生产燃料乙醇的工艺优化

Optimization of Technique for Producing Fuel Ethanol by Cassava Simultaneous Saccharification and Fermentation

[目的]优化木薯同步糖化发酵生产燃料乙醇的工艺。[方法]在实验室进行发酵试验,通过前期单因素试验的结果选取了3个对发酵结果影响较大的因素:发酵时间、发酵温度、料水比按正交试验设计方案进行发酵条件的优化。[结果]试验表明,3个因素对木薯同步糖化生产燃料乙醇的影响作用的主次顺序为发酵时间、发酵温度、料水比;优化方案为:料水比1∶2.5 g/ml,发酵温度32℃,发酵时间54 h,优化后的发酵结果酒度达到了14.3%(V/V),原料转化率达到了30.7%。[结论]研究可为木薯发酵生产燃料乙醇提供理论依据。

[Objective] To optimize technique for producing fuel ethanol by cassava simultaneous saccharification and fermentation.[Method] Three main influencing factors selected from single factor experiment,including fermentation time,temperature and solid-liquid ratio,were used to conduct the orthogonal test,so as to optimize the fermentation conditions.[Result] The experiment showed that,the order of three factors influencing ethanol production is fermentation time ＞ temperature ＞ solid-liquid ratio.The optimal scheme is：solid-liquid ratio 1 ∶ 2.5 g/ml,fermentation temperature 32 ℃,fermentation time 54 h,the concentration of ethanol is up to 14.3％ （V/V）,material conversion rate reaches 30.7％.[Conclusion] The study can provide theoretical basis for producing fuel ethanol by cassava fermentation.