摘要
目的:采用代谢组学方法考察石榴皮鞣质对正常大鼠尿液内源性物质代谢的影响。方法:将大鼠分为空白对照组、石榴皮鞣质给药组,每组按78.5 mg·ml-1剂量对大鼠连续灌胃给药4周,收集尿样,用于代谢组学研究。HPLC-MS采集大鼠尿样的数据信息,运用SIMCA-P软件中的PCA法与PLS-DA法对比分析。结果:石榴皮鞣质给药组与空白对照组得分点达到很好的区分效果,连续给药22 d时,尿液内源性物质代谢改变最为明显。结论:石榴皮鞣质提取物显著影响大鼠尿样中与药理作用相关内源性物质的代谢,为深入研究石榴皮鞣质提供依据。
Objective：To study the influence of pomegranate tannins on the endogenous metabolites in the urine of normal rats by metabonomics method. Methods：The rats were divided into two groups, the blank control group and tannins group. The rats in tan-nins group were orally administered 78. 5 mg·ml-1 pomegranate tannins for 4 weeks. The urine samples were collected and used in the metabonomics study. The urine data were collected by HPLC-MS and comparatively analyzed using PCA and PLS-DA method in SIM-CA-P software. Results：The score points of tannins group displayed obvious distance compared with those of the blank control group, and the changes were the most obvious on the 22nd administration day. Conclusion：Endogenous metabolites in rat urine are obviously influenced by pomegranate tannins, and the research provides the basis for the further study of pomegranate tannins.
出处
《中国药师》
CAS
2014年第10期1609-1612,共4页
China Pharmacist
基金
国家自然科学基金资助项目(编号:31170330)
关键词
石榴皮鞣质
给药时间
主成分分析
偏最小二乘法判别分析
Pomegranate tannins
Administration time
Principal component analysis
Partial least squares-discriminate analysis
