银杏总黄酮对心力衰竭大鼠心功能及心肌组织超氧化物歧化酶、丙二醛含量的影响研究

Effect of Gingko Flavonoids on Cardiac Function and Contents of SOD and MDA in Myocardial Tissue in Rats with Heart Failure

目的:探讨银杏总黄酮对心力衰竭大鼠心功能及心肌组织超氧化物歧化酶(SOD)和丙二醛(MDA)含量变化的影响。方法:SPF级雄性SD大鼠40只,随机分为5组:空白组、模型组、银杏总黄酮低剂量组、高剂量组和美托洛尔组。除空白组外其余均给予皮下注射异丙基肾上腺素300 mg·kg-1·d-1,持续2 d,造模成功后美托洛尔组给予美托洛尔20 mg·kg-1·d-1,银杏总黄酮高低剂量组分别给予500、250 mg·kg-1·d-1的银杏总黄酮,空白组和模型组灌胃给予相同体积的生理盐水,共给药8周。于给药8周后测定大鼠心功能指标包括室间隔厚度(IVST)、左心室后壁厚度(LVPWT)、左心室舒张末内径(LVEDD)、左心室收缩末内径(LVESD)和左心室射血百分数(LVEF),心肌组织SOD、MDA含量和病理积分,并取心肌组织染色观察病理形态学特征。结果:给药8周后,银杏总黄酮组高、低剂量组和美托洛尔组LVEF、IVST和LVPWT降低,LVEDD、LVESD升高,与模型组比较差异显著(P<0.05);银杏总黄酮高、低剂量组SOD水平上升,MDA含量和病理评分明显下降,与模型组比较差异有统计学意义(P<0.05),且随剂量加大有剂量依赖性趋势。结论:银杏总黄酮对心衰大鼠的心功能具有一定的保护作用,其作用机制可能与降低脂质过氧化水平,提高机体抗氧化酶活性有关。

Objective：To explore the effect of gingko flavonoids on the content of SOD and MDA and cardiac function in the rats with heart failure. Methods：Totally 40 male Sprague-Dawley rats were randomly divided into 5 groups by random number table meth-od, namely the blank group, the model group, low-dose gingko flavonoids group, high-dose gingko flavonoids group and metoprolol group. Except the blank group, all the other groups were subcutaneously injected 300 mg·kg-1 isopropyl adrenaline for two days to make the heart failure model. After that, metoprolol group was given metoprolol 20 mg·kg^-1 ·d^-1 , the ginkgo flavonoids group was given 250 or 500 mg·kg^-1 ·d^-1 ginkgo flavonoids extract, the other groups were given physiological saline with the same volume, and the treatment course was 8 weeks. After the treatment, cardiac function indices （ IVST, LVPWT, LVEDD, LVESD and LVEF） , SOD and MDA levels in cardiac muscle tissue and the pathological integral, and myocardial pathologic morphology characteristics were deter-mined. Results：LVEDD and LVESD of the two ginkgo flavonoids groups and metoprolol group were obviously improved compared with those of the model group, while LVEF, IVST and LVPWT were significantly decreased （P〈0. 05）, showing that the cardiac function was improved after the treatment. The levels of SOD were raised, while MDA and the pathological integral were decreased significantly in the two ginkgo flavonoids groups compared with those of the model group （P〈0. 05）, with a dose-dependant relationship. Conclu-sion：Ginkgo flavonoids have certain protective effect in heart failure rats, and the mechanism may be related to reducing the level of lipid peroxidation and improving the body antioxidant enzyme activity.