复方门冬维甘滴眼液中苯扎氯铵的测定

Determination of Benzalkonium Chloride in Compound Aspartate,Vitamin B_6 and Dipotassium Glycyrrhetate Eye Drops

目的：建立测定复方门冬维甘滴眼液中苯扎氯铵含量的方法。方法：采用Agilent Zorbax C18色谱柱（150 mm ×4.6 mm,5μm）,以乙腈-5 mmol·L^-1醋酸铵溶液（含1%三乙胺,用冰醋酸调节pH至5.0＆#177;0.5）（65∶35）为流动相,流速为1.0 ml·min^-1,检测波长为214 nm。结果：苯扎氯铵在50.00~150.00μg·ml^-1范围内与峰面积线性关系良好,r=0.9980；平均回收率为99.1%,RSD为0.6%。结论：该方法简便,迅速,灵敏度高,具有良好的重复性及回收率,可用于复方门冬维甘滴眼液中苯扎氯铵的测定。

Objective：To develop a method to determine benzalkonium chloride in compound aspartate, vitamin B6 and dipotassi-umglycyrrhetateeyedrops.Methods：TheseparationwasperformedonanAgilentZorbaxC18（150mm×4.6mm,5μm）column. The mobile phase consisted of acetonitrile-5 mmol·L^-1 ammonium acetate （ in which 1% triethylamine was added, then the pH value was adjusted to 5. 0 ＆#177; 0. 5 by acetic acid） 65∶35（v/v）. The flow rate was 1. 0 ml·min^-1 and the detection wavelength was 214nm. Re-sults：Benzalkonium chloride had a good linearity within the range of 50. 00-150. 00μg·ml-1（r=0. 998 0）, and the average recovery was 99. 1%（RSD=0. 6%）. Conclusion：The method is simple, rapid and sensitive with good repeatability and recovery. It can be used to determine benzalkoinum chloride in compound aspartate, vitamin B6 and dipotassium glycyrrhetate eye drops.