摘要
目的:建立测定复方门冬维甘滴眼液中苯扎氯铵含量的方法。方法:采用Agilent Zorbax C18色谱柱(150 mm ×4.6 mm,5μm),以乙腈-5 mmol·L^-1醋酸铵溶液(含1%三乙胺,用冰醋酸调节pH至5.0±0.5)(65∶35)为流动相,流速为1.0 ml·min^-1,检测波长为214 nm。结果:苯扎氯铵在50.00~150.00μg·ml^-1范围内与峰面积线性关系良好,r=0.9980;平均回收率为99.1%,RSD为0.6%。结论:该方法简便,迅速,灵敏度高,具有良好的重复性及回收率,可用于复方门冬维甘滴眼液中苯扎氯铵的测定。
Objective:To develop a method to determine benzalkonium chloride in compound aspartate, vitamin B6 and dipotassi-umglycyrrhetateeyedrops.Methods:TheseparationwasperformedonanAgilentZorbaxC18(150mm×4.6mm,5μm)column. The mobile phase consisted of acetonitrile-5 mmol·L^-1 ammonium acetate ( in which 1% triethylamine was added, then the pH value was adjusted to 5. 0 ± 0. 5 by acetic acid) 65∶35(v/v). The flow rate was 1. 0 ml·min^-1 and the detection wavelength was 214nm. Re-sults:Benzalkonium chloride had a good linearity within the range of 50. 00-150. 00μg·ml-1(r=0. 998 0), and the average recovery was 99. 1%(RSD=0. 6%). Conclusion:The method is simple, rapid and sensitive with good repeatability and recovery. It can be used to determine benzalkoinum chloride in compound aspartate, vitamin B6 and dipotassium glycyrrhetate eye drops.
出处
《中国药师》
CAS
2014年第10期1778-1779,共2页
China Pharmacist
关键词
苯扎氯铵
高效液相色谱法
复方门冬维甘滴眼液
含量测定
Benzalkonium chloride
HPLC
Compound aspartate,vitamin B6 and dipotassium glycyrrhetate eye drops
Determination