HPLC测定大叶金花草中牡荆素的含量

Determination of Viterxin in Stenoloma Chusana( L. ) Citing

目的：建立高效液相色谱法测定大叶金花草中牡荆素含量。方法：采用Waters Symmetry C18（250 mm ×4.6 mm,5μm）色谱柱,流动相为乙腈-0.1%冰乙酸溶液（27∶73）,流速为1.0 ml·min^-1,检测波长为339 nm,柱温为30℃。结果：牡荆素浓度在10.03~90.27μg·ml^-1范围内与峰面积的线性关系良好（r=0.9999）,平均回收率为100.48%,RSD为0.98%。结论：本分析方法准确、灵敏、重现性好,可用于大叶金花草中牡荆素的含量测定。

Objective：To establish an HPLC method for the content determination of viterxin in Stenoloma chusana（ L. ） Citing. Methods：A Waters Symmetry C18 （250 mm × 4. 6 mm, 5 μm） column was used, and the mobile phase was acetonitrile-0. 1% glacial acetic acid solution （27∶73） at the flow rate of 1. 0 ml·min^-1 . The detection wavelength was at 339nm, and the column temperature was 30℃. Results：The linear relationship of viterxin was good within the range from 10. 03 to 90. 27μg·ml^-1 . The average recovery was 100. 48%（RSD=0. 98%）. Conclusion：The established method is accurate and sensitive with good reproducibility, which can be applied in the determination of viterxin of Stenoloma chusana（ L. ） Citing.